ArticleApplied and environmental microbiology1996
Analysis of heterologous protein production in defined recombinant Aspergillus awamori strains.
Article in Applied and environmental microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 86 citations in OpenAlex.
- An overview on current molecular tools for heterologous gene expression in Trichoderma.Fungal biology and biotechnology · 2021Review
- Characterization of the effects of terminators and introns on recombinant gene expression in the basidiomycete Ceriporiopsis subvermispora.Journal of microbiology (Seoul, Korea) · 2020Article
- Cloning and Expression of Gumboro VP2 Antigen in Aspergillus niger.Avicenna journal of medical biotechnology · 2013Article
- Repeated-batch production of glucoamylase using recombinant Saccharomyces cerevisiae immobilized in a fibrous bed bioreactor.Journal of industrial microbiology & biotechnology · 2010Article
- Codon optimization increases steady-state mRNA levels in Aspergillus oryzae heterologous gene expression.Applied and environmental microbiology · 2008Article
- Improvement of foreign-protein production in Aspergillus niger var. awamori by constitutive induction of the unfolded-protein response.Applied and environmental microbiology · 2003Article
- High-yield production of a bacterial xylanase in the filamentous fungus Trichoderma reesei requires a carrier polypeptide with an intact domain structure.Applied and environmental microbiology · 2003Article
- Evaluation of Aspergillus niger as host for virus-like particle production, using the hepatitis B surface antigen as a model.Current genetics · 2003Article
- Effect of introns and AT-rich sequences on expression of the bacterial hygromycin B resistance gene in the basidiomycete Schizophyllum commune.Applied and environmental microbiology · 2001Article
- Characterization of a foldase, protein disulfide isomerase A, in the protein secretory pathway of Aspergillus niger.Applied and environmental microbiology · 2000Article
- Filamentous fungi as production organisms for glycoproteins of bio-medical interest.Glycoconjugate journal · 1999Review
- Expression and secretion of defined cutinase variants by Aspergillus awamori.Applied and environmental microbiology · 1998Article
- Glucoamylase gene fusions alleviate limitations for protein production in Aspergillus awamori at the transcriptional and (post) translational levels.Applied and environmental microbiology · 1997Article
- Kinetics of mRNA and protein synthesis of genes controlled by the 1,4-beta-endoxylanase A promoter in controlled fermentations of Aspergillus awamori.Applied and environmental microbiology · 1996Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A study was carried out to obtain more insight into the parameters that determine the secretion of heterologous proteins from filamentous fungi. A strategy was chosen in which the mRNA levels and protein levels of a number of heterologous genes of different origins were compared. All genes were under control of the Aspergillus awamori 1,4-beta-endoxylanase A (exlA) expression signals and were integrated in a single copy at the A. awamori pyrG locus. A Northern (RNA) analysis showed that large differences occurred in the steady-state mRNA levels obtained with the various genes; those levels varied from high values for genes of fungal origin (A. awamori 1,4-beta-endoxylanase A, Aspergillus niger glucoamylase, and Thermomyces lanuginosa lipase) to low values for genes of nonfungal origin (human interleukin 6 and Cyamopsis tetragonoloba [guar] alpha-galactosidase). With the C. tetragonoloba alpha-galactosidase wild-type gene full-length mRNA was even undetectable. Surprisingly, small amounts of full-length mRNA could be detected when a C. tetragonoloba alpha-galactosidase gene with an optimized Saccharomyces cerevisiae codon preference was expressed. In all cases except human interleukin 6, the protein levels corresponded to the amounts expected on basis of the mRNA levels. For human interleukin 6, very low protein levels were observed, whereas relatively high steady-state mRNA levels were obtained. Our data suggest that intracellular protein degradation is the most likely explanation for the low levels of secreted human interleukin 6.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.