ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1996
Whole-cell chloride currents in rat astrocytes accompany changes in cell morphology.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 80 citations in OpenAlex.
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- Altered Mechanical Properties of Astrocytes Lacking MLC1: Implications for the Leukodystrophy MLC.Glia · 2026Article
- Physiology of Astroglia.Physiological reviews · 2018Review
- Enteric neuropathy can be induced by high fat diet in vivo and palmitic acid exposure in vitro.PloS one · 2013Article
- A silk platform that enables electrophysiology and targeted drug delivery in brain astroglial cells.Biomaterials · 2010Article
- Astrocytic plasticity and patterned oxytocin neuronal activity: dynamic interactions.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Effect of P2 receptor on the intracellular calcium increase by cancer cells in human umbilical vein endothelial cells.Naunyn-Schmiedeberg's archives of pharmacology · 2008Article
- High extracellular K(+) evokes changes in voltage-dependent K(+) and Na (+) currents and volume regulation in astrocytes.Pflugers Archiv : European journal of physiology · 2007Article
- Article
- Expression of voltage-gated chloride channels in human glioma cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Volume-activated chloride currents contribute to the resting conductance and invasive migration of human glioma cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Article
- Ca2+-sensing receptor-mediated regulation of volume-sensitive Cl- channels in human epithelial cells.The Journal of physiology · 2000Article
- Glutamate release through volume-activated channels during spreading depression.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Modulation of glioma cell migration and invasion using Cl(-) and K(+) ion channel blockers.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Involvement of stretch-activated Cl- channels in ramification of murine microglia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Prostaglandin E2 and 4-aminopyridine prevent the lipopolysaccharide-induced outwardly rectifying potassium current and interleukin-1beta production in cultured rat microglia.Journal of neurochemistry · 1998Article
- Cytoskeletal actin gates a Cl- channel in neocortical astrocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Mitogen-activated protein and tyrosine kinases in the activation of astrocyte volume-activated chloride current.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Astrocytes can change shape dramatically in response to increased physiological and pathological demands, yet the functional consequences of morphological change are unknown. We report the expression of Cl- currents after manipulations that alter astrocyte morphology. Whole-cell Cl- currents were elicited after (1) rounding up cells by brief exposure to trypsin; (2) converting cells from a flat polygonal to a process-bearing (stellate) morphology by exposure to serum-free Ringer's solution; and (3) swelling cells by exposure to hypo-osmotic solution. Zero-current potentials approximated the Nernst for Cl-, and rectification usually followed that predicted by the constant-field equation. We observed heterogeneity in the activation and inactivation kinetics, as well as in the relative degree of outward versus inward rectification. Cl- conductances were inhibited by 4, 4-diisothiocyanostilbene-2,2'-disulfonic acid (200 microM) and by Zn2+ (1 mM). Whole-cell Cl- currents were not expressed in cells without structural change. We investigated whether changes in cytoskeletal actin accompanying changes in astrocytic morphology play a role in the induction of shape-dependent Cl- currents. Cytochalasins, which disrupt actin polymers by enhancing actin-ATP hydrolysis, elicited whole-cell Cl- conductances in flat, polygonal astrocytes. In stellate cells, elevated intracellular Ca2+ (2 microM), which can depolymerize actin, enhanced Cl- currents, and high intracellular ATP (5 mM), required for repolymerization, reduced Cl- currents. Modulation of Cl- current by Ca2+ and ATP was blocked by concurrent whole-cell dialysis with phalloidin and DNase, respectively. Phalloidin stabilizes actin polymers and DNase inhibits actin polymerization. Dialysis with phalloidin also prevented hypo-osmotically activated Cl- currents. These results demonstrate how the expression of astrocyte Cl- currents can be dependent on cell morphology, the structure of actin, Ca2+ homeostasis, and metabolism.
Indexed as
Identifiers
8786429PMC2695502W1961610463What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.