Evidence map›Paper›PMID 8785605›Full record

ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology1996

Chromosome-specific paints from a high-resolution flow karyotype of the dog.

C F Langford, P E Fischer, M M Binns, N G Holmes, N P Carter

Abstract read
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In one paragraph

Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
18.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 91 citations in OpenAlex.

  1. Article
  2. Article
  3. Chromosomes in the flow to simplify genome analysis.Functional & integrative genomics · 2012
    Review
  4. Article
  5. Article
  6. Article
  7. Canine cytogenetics--from band to basepair.Cytogenetic and genome research · 2008
    Review
  8. Tackling the characterization of canine chromosomal breakpoints with an integrated in-situ/in-silico approach: the canine PAR and PAB.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2008
    Article
  9. Article
  10. Review
  11. High-speed chromosome sorting.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2004
    Review
  12. Karyotype of canine soft tissue sarcomas: a multi-colour, multi-species approach to canine chromosome painting.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2004
    Article
  13. Article
  14. A 1-Mb resolution radiation hybrid map of the canine genome.Proceedings of the National Academy of Sciences of the United States of America · 2003
    Article
  15. Article
  16. Article
  17. Molecular cytogenetic analysis of a novel high-grade canine T-lymphoblastic lymphoma demonstrating co-expression of CD3 and CD79a cell markers.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2001
    Article
  18. A comparative chromosome map of the Arctic fox, red fox and dog defined by chromosome painting and high resolution G-banding.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2000
    Article
  19. Chromosome identification and assignment of DNA clones in the dog using a red fox and dog comparative map.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2000
    Article
  20. The DAPI banded karyotype of the domestic dog (Canis familiaris) generated using chromosome-specific paint probes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1999
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

C F LangfordSanger Centre, Hinxton, Cambridge, UK.
P E Fischer
M M Binns
N G Holmes
N P Carter
Animal Health Trust · GBWellcome Sanger Institute · GB

Funding

Wellcome Trust
6 · The paper itself

Abstract

Using peripheral blood lymphocyte cultures and dual-laser flow cytometry, we have routinely obtained high-resolution bivariate flow karyotypes of the dog in which 32 peaks are resolved. To allow the identification of the chromosome types in each peak, chromosomes were flow sorted, amplified and labelled by polymerase chain reaction with partially degenerate primers and hybridized onto metaphase spreads of a male dog. The chromosome paints from 22 of the 32 peaks each hybridized to single homologue pairs and eight peaks each hybridized to two pairs. Paints from the remaining two peaks hybridized to only one homologue each in the male metaphase spread, thus corresponding to the sex chromosomes X and Y. All of the 38 pairs of autosomes and the two sex chromosomes of the dog could be accounted for in these painting experiments. The positions of chromosomes 1-21 were assigned to the flow karyotype (only chromosomes 1-21 have as yet been officially designated). The high-resolution flow karyotype and the chromosome paints will facilitate further standardization of the dog karyotype. The ability to sort sufficient quantities of dog chromosomes for the production of chromosome-specific DNA libraries has the potential to accelerate the physical and genetic mapping of the dog genome.

Indexed as

Chromosome BandingIn Situ Hybridization, FluorescenceKaryotypingAnimalsBase SequenceBiotinChromomycinsChromosomesDNA PrimersDogsFemaleFlow CytometryLymphocytesMaleMolecular Sequence DataPolymerase Chain ReactionBiotinChromomycinsDNA Primers

Identifiers

PMID8785605
OpenAlexW2056897529

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.