ArticleBiophysical journal1996
The effects of external pH on calcium channel currents in bullfrog sympathetic neurons.
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Neural Processing without OPhysiology (Bethesda, Md.) · 2024Review
- Altered functional properties of the codling moth Orco mutagenized in the intracellular loop-3.Scientific reports · 2021Article
- Two central pattern generators from the crab,eLife · 2018Article
- YC‑1 reduces inflammatory responses by inhibiting nuclear factor‑κB translocation in mice subjected to transient focal cerebral ischemia.Molecular medicine reports · 2018Article
- Low pHThe Journal of physiology · 2017Article
- Ca2+ removal by the plasma membrane Ca2+-ATPase influences the contribution of mitochondria to activity-dependent Ca2+ dynamics in Aplysia neuroendocrine cells.Journal of neurophysiology · 2016Article
- Proton-mediated block of Ca2+ channels during multivesicular release regulates short-term plasticity at an auditory hair cell synapse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Acidification of the synaptic cleft of cone photoreceptor terminal controls the amount of transmitter release, thereby forming the receptive field surround in the vertebrate retina.The journal of physiological sciences : JPS · 2012Review
- Mitochondrial imaging in dorsal root ganglion neurons following the application of inducible adenoviral vector expressing two fluorescent proteins.Journal of neuroscience methods · 2008Article
- pH changes in the invaginating synaptic cleft mediate feedback from horizontal cells to cone photoreceptors by modulating Ca2+ channels.The Journal of general physiology · 2003Article
- Extracellular Ca2+ modulates the effects of protons on gating and conduction properties of the T-type Ca2+ channel alpha1G (CaV3.1).The Journal of general physiology · 2003Article
- Monovalent cations contribute to T-type calcium channel (Cav3.1 and Cav3.2) selectivity.The Journal of membrane biology · 2003Article
- Paracellular ion channel at the tight junction.Biophysical journal · 2003Article
- The influence of plasma membrane electrostatic properties on the stability of cell ionic composition.Biophysical journal · 2001Article
- pH modification of human T-type calcium channel gating.Biophysical journal · 2000Article
- Acid-evoked quantal catecholamine secretion from rat phaeochromocytoma cells and its interaction with hypoxia-evoked secretion.The Journal of physiology · 1999Article
- Proton and zinc effects on HERG currents.Biophysical journal · 1999Article
- Rapid and direct effects of pH on connexins revealed by the connexin46 hemichannel preparation.The Journal of general physiology · 1999Article
- Overview of voltage-dependent calcium channels.Journal of bioenergetics and biomembranes · 1998Review
- Surface charge and lanthanum block of calcium current in bullfrog sympathetic neurons.Biophysical journal · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
We have investigated the effects of external pH (pHo) on whole-cell calcium channel currents in bullfrog sympathetic neurons. The peak inward current increased at alkaline pHo and decreased at acidic pHo. We used tail currents to distinguish effects of pHo on channel gating and permeation. There were large shifts in the voltage dependence of channel activation (approximately 40 mV between pHo and 9.0 and pHo 5.6), which could be explained by binding of H+ to surface charge according to Gouy-Chapman theory. To examine the effects of pHo on permeation, we measured tail currents at 0 mV, following steps to + 120 mV to maximally activate the channels. Unlike most previous studies, we found only a approximately 10% reduction in channel conductance from pHo 9.0 to pHo 6.4, despite a approximately 25 mV shift of channel activation. At lower pHo the channel conductance did decrease, which could be described by binding of H+ to a site with pKa = 5.1. In some cells, there was a separate slow decrease in conductance at low pHo, possibly because of changes in internal pH. These results suggest that changes in current at pHo > 6.4 result primarily from a shift in the voltage dependence of channel activation. A H(+)-binding site can explain a rapid decrease in channel conductance at lower pHo. The surface charge affecting gating has little effect on the local ion concentration near the pore, or on the channel conductance.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.