ArticleMolecular medicine (Cambridge, Mass.)1996
Insulin-stimulated glucose uptake involves the transition of glucose transporters to a caveolae-rich fraction within the plasma membrane: implications for type II diabetes.
Article in Molecular medicine (Cambridge, Mass.), 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 103 citations in OpenAlex.
- Statin Use and Hyperglycemia: Do Statins Cause Diabetes?Current atherosclerosis reports · 2024Review
- Role of Gut Microbiota in Statin-Associated New-Onset Diabetes-A Cross-Sectional and Prospective Analysis of the FINRISK 2002 Cohort.Arteriosclerosis, thrombosis, and vascular biology · 2024Article
- EHD2 regulates plasma membrane integrity and downstream insulin receptor signaling events.Molecular biology of the cell · 2023Article
- The importance of caveolin as a target in the prevention and treatment of diabetic cardiomyopathy.Frontiers in immunology · 2022Review
- Updated Understanding of the Crosstalk Between Glucose/Insulin and Cholesterol Metabolism.Frontiers in cardiovascular medicine · 2022Review
- Drugs Interfering with Insulin Resistance and Their Influence on the Associated Hypermetabolic State in Severe Burns: A Narrative Review.International journal of molecular sciences · 2021Review
- Statin Treatment-Induced Development of Type 2 Diabetes: From Clinical Evidence to Mechanistic Insights.International journal of molecular sciences · 2020Review
- Mechanisms of statin-induced new-onset diabetes.Journal of cellular physiology · 2019Review
- Sterol structure dependence of insulin receptor and insulin-like growth factor 1 receptor activation.Biochimica et biophysica acta. Biomembranes · 2019Article
- Cholesterol-enriched membrane microdomains are needed for insulin signaling and proliferation in hepatic cells.American journal of physiology. Gastrointestinal and liver physiology · 2018Article
- Leucaena leucocephala fruit aqueous extract stimulates adipogenesis, lipolysis, and glucose uptake in primary rat adipocytes.TheScientificWorldJournal · 2014Article
- Insulin-stimulated glucose uptake occurs in specialized cells within the cumulus oocyte complex.Endocrinology · 2012Article
- Insulin controls the spatial distribution of GLUT4 on the cell surface through regulation of its postfusion dispersal.Cell metabolism · 2010Article
- Resveratrol enhances GLUT-4 translocation to the caveolar lipid raft fractions through AMPK/Akt/eNOS signalling pathway in diabetic myocardium.Journal of cellular and molecular medicine · 2008Article
- Dissociation of the insulin receptor and caveolin-1 complex by ganglioside GM3 in the state of insulin resistance.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Compartmentalization of the exocyst complex in lipid rafts controls Glut4 vesicle tethering.Molecular biology of the cell · 2006Article
- Spatial and temporal regulation of GLUT4 translocation by flotillin-1 and caveolin-3 in skeletal muscle cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2006Article
- Reduced insulin-stimulated GLUT4 bioavailability in stroke-prone spontaneously hypertensive rats.Diabetologia · 2005Article
- Structure-function analysis of the presumptive Arabidopsis auxin permease AUX1.The Plant cell · 2004Article
- Isoform-specific insulin receptor signaling involves different plasma membrane domains.The Journal of cell biology · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAdipose and muscle tissues express an insulin-sensitive glucose transporter (GLUT4). This transporter has been shown to translocate from intracellular stores to the plasma membrane following insulin stimulation. The molecular mechanisms signalling this event and the details of the translocation pathway remain unknown. In type II diabetes, the cellular transport of glucose in response to insulin is impaired, partly explaining why blood-glucose levels in patients are not lowered by insulin as in normal individuals. MATERIALS AND
methodsIsolated rat epididymal adipocytes were stimulated with insulin and subjected to subcellular fractionation and to measurement of glucose uptake. A caveolae-rich fraction was isolated from the plasma membranes after detergent solubilization and ultracentrifugal floatation in a sucrose gradient. Presence of GLUT4 and caveolin was determined by immunoblotting after SDS-PAGE.
resultsIn freshly isolated adipocytes, insulin induced a rapid translocation of GLUT4 to the plasma membrane fraction, which was followed by a slower transition of the transporter into a detergent resistant caveolae-rich region of the plasma membrane. The insulin-stimulated appearance of transporters in the caveolae-rich fraction occurred in parallel with enhanced glucose uptake by cells. Treatment with isoproterenol plus adenosine deaminase rapidly inhibited insulin-stimulated glucose transport by 40%, and at the same time GLUT4 disappeared from the caveolae-rich fraction and from plasma membranes as a whole.
conclusionsInsulin stimulates glucose uptake in adipocytes by rapidly translocating GLUT4 from intracellular stores to the plasma membrane. This is followed by a slower transition of GLUT4 to the caveolae-rich regions of the plasma membrane, where glucose transport appears to take place. These results have implications for an understanding of the defect in glucose transport involved in type II diabetes.
Indexed as
Identifiers
8784789PMC2230151W1583163003What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.