ArticleHistochemistry and cell biology1996
Cytoskeletal markers allowing discrimination between brush cells and other epithelial cells of the gut including enteroendocrine cells.
Article in Histochemistry and cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 99 citations in OpenAlex.
- Differential expression of villin and advillin by neuroendocrine and tuft cells in the murine lower airways.Cell and tissue research · 2025Article
- Regulation of the tuft cell-ILC2 circuit in intestinal mucosal immunity.Frontiers in immunology · 2025Review
- Organization of a cytoskeletal superstructure in the apical domain of intestinal tuft cells.The Journal of cell biology · 2024Article
- Intestinal Tuft Cells Are Enriched With Protocadherins.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2024Article
- Intestinal Tuft Cells: Morphology, Function, and Implications for Human Health.Annual review of physiology · 2024Review
- The morphological and functional diversity of apical microvilli.Journal of anatomy · 2023Review
- Possible connection between intestinal tuft cells, ILC2s and obesity.Frontiers in immunology · 2023Review
- Novel protocol to observe the intestinal tuft cell using transmission electron microscopy.Biology open · 2022Article
- An update on the biological characteristics and functions of tuft cells in the gut.Frontiers in cell and developmental biology · 2022Review
- Tuft cell integration of luminal states and interaction modules in tissues.Pflugers Archiv : European journal of physiology · 2021Review
- Thymic epithelial cell heterogeneity: TEC by TEC.Nature reviews. Immunology · 2020Review
- Distribution pattern and molecular signature of cholinergic tuft cells in human gastro-intestinal and pancreatic-biliary tract.Scientific reports · 2019Article
- Interpreting heterogeneity in intestinal tuft cell structure and function.The Journal of clinical investigation · 2018Review
- Immunohistochemical characterization of brush cells in the rat larynx.Journal of molecular histology · 2018Article
- Dclk1-expressing tuft cells: critical modulators of the intestinal niche?American journal of physiology. Gastrointestinal and liver physiology · 2017Review
- Chemosensory epithelial cells in the urethra: sentinels of the urinary tract.Histochemistry and cell biology · 2016Review
- Intestinal tuft cells: epithelial sentinels linking luminal cues to the immune system.Mucosal immunology · 2016Review
- Tropomodulin 1 Regulation of Actin Is Required for the Formation of Large Paddle Protrusions Between Mature Lens Fiber Cells.Investigative ophthalmology & visual science · 2016Article
- Cholinergic epithelial cell with chemosensory traits in murine thymic medulla.Cell and tissue research · 2014Article
- Receptors for short-chain fatty acids in brush cells at the "gastric groove".Frontiers in physiology · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brush cells are specialised epithelial cells scattered throughout the simple epithelia of the respiratory and alimentary tracts. These cells have been suggested to serve a still unknown receptive function and use nitric oxide as a gaseous messenger molecule. At the light microscope level, brush cells can be identified by antibodies against the actin filament crosslinking proteins villin and fimbrin that not only stain the apical tuft of microvilli and their rootlets, but also label projections emanating from the basolateral surface of these cells. Since brush cells contain numerous intermediate filaments and microtubules and display a complicated basolateral cell morphology, we tested in this study whether antibodies against cytokeratin, tubulin and components of the membrane cytoskeleton might provide further markers for these cells at the light microscope level. Here we show that brush cells (identified by villin antibodies) can be discriminated from the neighbouring simple epithelium of the stomach, pancreatic duct and duodenum by particularly strong immunoreactivity with antibodies specific for cytokeratin 18. Tubulin antibodies reacted strongly with the upper half of brush cells in a pattern not observed in the other epithelial cells of these tissues, including enteroendocrine cells of the duodenum. Ankyrin, a protein that links the spectrin-based membrane cytoskeleton to integral proteins of the plasma membrane was revealed as a third cytoskeleton-associated protein, prominently expressed in brush cells where ankyrin is restricted to the basolateral membrane domain. The apparently high concentration of cytokeratin 18, tubulin and ankyrin in brush cells suggests that these cytoskeletal proteins might play a role in the mechanical stability and polarised organisation of these putative receptor cells.
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