ArticleChromosoma1996
HSF recruitment and loss at most Drosophila heat shock loci is coordinated and depends on proximal promoter sequences.
Article in Chromosoma, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Molecular Chaperone Hsp70 and Its Constitutively Active Form Hsc70 Play an Indispensable Role During Eye Development of Drosophila melanogaster.Molecular neurobiology · 2018Article
- Fluorescent protein tagging confirms the presence of ribosomal proteins at Drosophila polytene chromosomes.PeerJ · 2013Article
- Recruitment of the ATP-dependent chromatin remodeler dMi-2 to the transcribed region of active heat shock genes.Nucleic acids research · 2012Article
- Article
- Chromatin landscape dictates HSF binding to target DNA elements.PLoS genetics · 2010Article
- Paused Pol II captures enhancer activity and acts as a potent insulator.Genes & development · 2009Article
- The effects of temperature shock on transcription and replication in Rhynchosciara americana (Diptera: Sciaridae).Genetica · 2006Article
- Genomic analysis of heat-shock factor targets in Drosophila.Genome biology · 2005Article
- Protein phosphatase 2A activity affects histone H3 phosphorylation and transcription in Drosophila melanogaster.Molecular and cellular biology · 2003Article
- Heterochromatin protein 1 (HP1) is associated with induced gene expression in Drosophila euchromatin.The Journal of cell biology · 2003Article
- Biological functions of the ISWI chromatin remodeling complex NURF.Genes & development · 2002Article
- Spt5 and spt6 are associated with active transcription and have characteristics of general elongation factors in D. melanogaster.Genes & development · 2000Article
- P-TEFb kinase recruitment and function at heat shock loci.Genes & development · 2000Article
- RNA aptamers as effective protein antagonists in a multicellular organism.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- TATA box-binding protein (TBP)-related factor 2 (TRF2), a third member of the TBP family.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- GAGA factor binding to DNA via a single trinucleotide sequence element.Nucleic acids research · 1998Article
- Dynamics of potentiation and activation: GAGA factor and its role in heat shock gene regulation.Nucleic acids research · 1997Review
- Involvement of general transcriptional factors in the regulation of transcription of the hsp70 gene in vivo.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sectionsArticle
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Authors and funding
2 authors.
Funding
Abstract
The heat shock response in Drosophila is primarily dependent on the binding of the heat shock transcription factor, HSF, to conserved sequences in heat shock gene promoters, the heat shock elements (HSEs). Here we examine the kinetic relationship of HSF binding to chromosomal loci and heat shock gene transcription in vivo. The features of heat shock promoters that determine the kinetics of HSF binding are also examined. Analyses of HSF association by indirect immunofluorescence with an anti-HSF antibody reveal that fluorescent signals at many loci on polytene chromosomes rapidly increase and then gradually decrease as heat shock time progresses. While overall amounts of fluorescent signal vary from locus to locus, the patterns of acquisition and loss of HSF at most loci are coordinated with only one identified exception. Immunostaining with an anti-RNA polymerase II antibody indicates that the kinetics of RNA polymerase II accumulation on the heat shock loci are similar to those of HSF. Furthermore, nuclear run-on assays confirm that the major heat shock genes are coordinately transcribed during the attenuation period. In contrast, the kinetics of HSF association with HSE "polymers" in a transgenic fly strain are not coordinated with those of endogenous loci. The addition of core promoter sequences to one of the HSEs found in the polymer restores coordinate HSF binding, suggesting that the kinetic patterns of HSF binding depend on a core promoter located near the HSEs. Finally, the distribution of the heat shock protein HSP70 is examined for its role in regulating the attenuated response of HSF to heat shock.
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