ArticleThe Plant cell1996
Nuclear import in permeabilized protoplasts from higher plants has unique features.
Article in The Plant cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Who cites it
20 citing papers in PubMed, 77 citations in OpenAlex.
- Disruption of nucleocytoplasmic trafficking as a cellular senescence driver.Experimental & molecular medicine · 2021Article
- Coordination of NMCP1- and NMCP2-class proteins within the plant nucleoskeleton.Molecular biology of the cell · 2020Article
- Suitable transfection methods for single particle tracing in plant suspension cells.Plant methods · 2014Article
- The Arabidopsis nuclear pore and nuclear envelope.The arabidopsis book · 2010Article
- The VirE3 protein of Agrobacterium mimics a host cell function required for plant genetic transformation.The EMBO journal · 2005Article
- Nucleo-cytoplasmic partitioning of proteins in plants: implications for the regulation of environmental and developmental signalling.Current genetics · 2003Review
- Arabidopsis transportin1 is the nuclear import receptor for the circadian clock-regulated RNA-binding protein AtGRP7.Plant molecular biology · 2003Article
- Signal- and importin-dependent nuclear targeting of the kidney anion exchanger 1-binding protein kanadaptin.The Biochemical journal · 2002Article
- The balance of nuclear import and export determines the intracellular distribution and function of tomato heat stress transcription factor HsfA2.Molecular and cellular biology · 2001Article
- Import of Agrobacterium T-DNA into plant nuclei: two distinct functions of VirD2 and VirE2 proteins.The Plant cell · 2001Article
- Nuclear export in plants. Use of geminivirus movement proteins for a cell-based export assay.The Plant cell · 1999Article
- Matrix attachment region binding protein MFP1 is localized in discrete domains at the nuclear envelope.The Plant cell · 1999Article
- Nuclear localization signal receptor importin alpha associates with the cytoskeleton.The Plant cell · 1998Article
- Pleiotropic control of glucose and hormone responses by PRL1, a nuclear WD protein, in Arabidopsis.Genes & development · 1998Article
- The nuclear pore complex.Plant molecular biology · 1998Review
- A glycoprotein modified with terminal N-acetylglucosamine and localized at the nuclear rim shows sequence similarity to aldose-1-epimerases.The Plant cell · 1998Article
- Nuclear localization signal binding protein from Arabidopsis mediates nuclear import of Agrobacterium VirD2 protein.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Importin alpha from Arabidopsis thaliana is a nuclear import receptor that recognizes three classes of import signals.Plant physiology · 1997Article
- Characterization of AtSEC12 and AtSAR1. Proteins likely involved in endoplasmic reticulum and Golgi transport.Plant physiology · 1997Article
- Oilseed isocitrate lyases lacking their essential type 1 peroxisomal targeting signal are piggybacked to glyoxysomes.The Plant cell · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The import of proteins into the nucleus is a poorly understood process that is thought to require soluble cytosolic factors in vertebrates and yeast. To test this model in plants and to identify components of the import apparatus, we developed a direct in vitro nuclear import assay by using tobacco protoplasts that were permeabilized without detergents such as digitonin or Triton X-100. Substrates were imported specifically by a mechanism that required only guanine nucleotides. Moreover, in vitro import did not require exogenous cytosol. To investigate this novel finding, we isolated a full-length cDNA encoding an Arabidopsis homolog of vertebrate and yeast nuclear localization signal receptors and produced an affinity-purified antibody. The plant receptor was tightly associated with cellular components in permeabilized protoplasts, even in the presence of 0.1% Triton X-100, indicating that this factor and probably others were retained to an extent sufficient to support import. The lectin wheat germ agglutinin bound to the nucleus; however, it did not block translocation in our system, indicating that direct interaction with polysaccharide modifications at the nuclear pore complex was probably not essential for import in plants. Other features of in vitro import included reduced but significant import at low temperature.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.