ArticleNucleic acids research1996
Identification of in vivo target RNA sequences bound by thymidylate synthase.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Article
- Regulatory mechanisms of one-carbon metabolism enzymes.The Journal of biological chemistry · 2023Review
- Detection of a G-Quadruplex as a Regulatory Element inInternational journal of molecular sciences · 2020Article
- Cyclic Peptides Acting as Allosteric Inhibitors of Human Thymidylate Synthase and Cancer Cell Growth.Molecules (Basel, Switzerland) · 2019Article
- Topoisomerase I expression is associated with prognosis in postoperative non-small cell lung cancer patients.Thoracic cancer · 2016Article
- Article
- Translational control analysis by translationally active RNA capture/microarray analysis (TrIP-Chip).Nucleic acids research · 2010Article
- Identification and characterization of a novel thymidylate synthase from deep-sea thermophilic bacteriophage Geobacillus virus E2.Virus genes · 2008Article
- Antisense targeting of thymidylate synthase (TS) mRNA increases TS gene transcription and TS protein: effects on human tumor cell sensitivity to TS enzyme-inhibiting drugs.Gene expression · 2007Article
- Article
- Binding and repression of translation of the cognate mRNA by Trichinella spiralis thymidylate synthase differ from the corresponding interactions of the human enzyme.The Biochemical journal · 2005Article
- Nuclear thymidylate synthase expression, p53 expression and 5FU response in colorectal carcinoma.British journal of cancer · 2001Article
- Antisense-induced down-regulation of thymidylate synthase and enhanced cytotoxicity of 5-FUdR in 5-FUdR-resistant HeLa cells.British journal of pharmacology · 2001Article
- Effect of 2'-O-methyl antisense ORNs on expression of thymidylate synthase in human colon cancer RKO cells.Nucleic acids research · 2001Article
- Specific binding of Hoechst 33258 to site 1 thymidylate synthase mRNA.Nucleic acids research · 2000Article
- Antisense down-regulation of thymidylate synthase to suppress growth and enhance cytotoxicity of 5-FUdR, 5-FU and Tomudex in HeLa cells.British journal of pharmacology · 1999Article
- Regulation of p53 expression by thymidylate synthase.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Thymidylate synthase protein and p53 mRNA form an in vivo ribonucleoprotein complex.Molecular and cellular biology · 1999Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed an immunoprecipitation-RNA-random PCR (rPCR) method to isolate cellular RNA sequences that bind to the folate-dependent enzyme thymidylate synthase (TS). Using this approach, nine different cellular RNAs that formed a ribonucleoprotein (RNP) complex with thymidylate synthase (TS) in human colon cancer cells were identified. RNA binding experiments revealed that seven of these RNAs bound TS with relatively high affinity (IC50 values ranging from 1.5 to 6 nM). One of the RNAs was shown to encode the interferon (IFN)-induced 15 kDa protein. Western immunoblot analyses demonstrated that the level of IFN-induced 15 kDa protein was significantly decreased in human colon cancer H630-R10 cells compared with parent H630 cells. While the level of IFN-induced 15 kDa mRNA expression was the same in parent and TS-overexpressing cell lines, the level of IFN-induced 15 kDa RNA bound to TS in the form of a RNP complex was markedly higher in H630-R10 cells relative to parent H630 cells. These studies begin to define a number of cellular target RNA sequences with which TS interacts and suggest that these TS protein-cellular RNA interactions may have a biological role.
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