ArticleThe American journal of pathology1996
Dog pancreatic duct epithelial cells: long-term culture and characterization.
Article in The American journal of pathology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Pancreatic duct-like cell line derived from pig embryonic stem cells: expression of uroplakin genes in pig pancreatic tissue.In vitro cellular & developmental biology. Animal · 2019Article
- Development of a polarized pancreatic ductular cell epithelium for physiological studies.Journal of applied physiology (Bethesda, Md. : 1985) · 2018Article
- Extracellular ATP protects pancreatic duct epithelial cells from alcohol-induced damage through P2Y1 receptor-cAMP signal pathway.Cell biology and toxicology · 2016Article
- Contributions of protein kinases and β-arrestin to termination of protease-activated receptor 2 signaling.The Journal of general physiology · 2016Article
- Epithelial monolayer culture system for real-time single-cell analyses.Physiological reports · 2014Article
- Article
- Apoptotic damage of pancreatic ductal epithelia by alcohol and its rescue by an antioxidant.PloS one · 2013Article
- Actin cytoskeleton controls movement of intracellular organelles in pancreatic duct epithelial cells.Cell calcium · 2012Article
- Cyclic AMP potentiates Ca2+-dependent exocytosis in pancreatic duct epithelial cells.The Journal of general physiology · 2010Article
- An improved primary culture system of pancreatic duct epithelial cells from Wistar rats.Cytotechnology · 2009Article
- Control of granule mobility and exocytosis by Ca2+ -dependent formation of F-actin in pancreatic duct epithelial cells.Traffic (Copenhagen, Denmark) · 2009Article
- Protease-activated receptor-2 increases exocytosis via multiple signal transduction pathways in pancreatic duct epithelial cells.The Journal of biological chemistry · 2008Article
- Pattern of Ca2+ increase determines the type of secretory mechanism activated in dog pancreatic duct epithelial cells.The Journal of physiology · 2006Article
- Specific down-regulation of XIAP with RNA interference enhances the sensitivity of canine tumor cell-lines to TRAIL and doxorubicin.Molecular cancer · 2006Article
- Regulation of exocytosis by protein kinases and Ca(2+) in pancreatic duct epithelial cells.The Journal of general physiology · 2000Article
- Trypsin activates pancreatic duct epithelial cell ion channels through proteinase-activated receptor-2.The Journal of clinical investigation · 1999Article
- Culture of human main pancreatic duct epithelial cells.In vitro cellular & developmental biology. Animal · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epithelial cells, isolated from a normal dog pancreatic duct, were grown on collagen-coated culture inserts suspended above a feeder layer of myofibroblasts. The cells were examined by transmission electron microscopy, immunohistochemistry, cytogenetics, and flow cytometry. In addition, the constitutive and agonist-stimulated mucin secretion of these cells was studied using a [3H]N-acetyl-D-glucosamine labeling assay, and the stimulation of intracellular cAMP was measured. Cells grown on inserts with a feeder layer developed into confluent monolayers consisting of strictly polarized columnar epithelial cells with prominent microvilli, intercellular junctions, and normal chromosomal characteristics. They could be passaged repeatedly without a detectable alteration in their morphology. The cells could also be grown on organotypic cultures, resulting in further differentiated cells simulating in vivo morphology. Immunohistochemistry demonstrated the presence of carbonic anhydrase II in these cells. Cells treated with vasoactive intestinal peptide, epinephrine, and dibutyryl-cAMP demonstrated a marked increase in mucin secretion compared with controls. In parallel experiments, VIP and epinephrine significantly increased intracellular cAMP. In conclusion we have developed a pancreatic epithelial cell preparation with morphology, cytokinetics, chromosomal, and DNA analyses characteristic of normal cells. Similar to normal columnar epithelial cells, these pancreatic duct cells secreted mucin constitutively and responded to agonist by increasing secretion via a cAMP-mediated pathway. They also contained carbonic anhydrase, which indicates that the cells are capable of secreting bicarbonate.
Indexed as
Identifiers
8774152PMC1861705W2139549754What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.