ArticleBasic research in cardiology
Influence of phorbol esters on contractile force, action potential and calcium current of isolated guinea-pig heart tissues.
Article in Basic research in cardiology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed, 3 citations in OpenAlex.
- Regulation of ion channels in myocardial cells and protection of ischemic myocardium.Heart failure reviews · 2000Review
- Adrenergic control of the ultrarapid delayed rectifier current in canine atrial myocytes.The Journal of physiology · 1999Article
- alpha 1-adrenoceptor-mediated negative inotropy of adrenaline in rat myocardium.The Journal of physiology · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phorbol-12, 13-dibutyrate (PDB) reduced concentration-dependently the contractile force of guinea-pig papillary muscles (EC50 1.07 mumol/l) while phorbol-12-myristate-13-acetate (PMA) was ineffective. The protein kinase C inhibitors staurosporine (0.1 mumol/l) and polymyxin B (70 mumol/l) did not antagonize the negative inotropic effect of PDB. Neither PMA nor PDB, in concentrations up to 30 mumol/l, caused significant changes of the maximum depolarization velocity, the action potential duration or the functional refractory period in intact papillary muscles. In isolated ventricular cardiomyocytes the inward calcium current was halved by either 1 mumol/l PDB or 10 mumol/1 PMA. PKC inhibitors attenuated, but could not completely abolish this effect of the phorboles. It is concluded that the negative inotropic effect of PDB is caused by a reduction of the slow inward calcium current and that this inhibition is, for the greater part, not mediated by an activation of protein kinase C.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.