ArticlePflugers Archiv : European journal of physiology1996
A method for making rapid changes of superfusate whilst maintaining temperature at 37 degrees C.
Article in Pflugers Archiv : European journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Stereoselective block of the hERG potassium channel by the Class Ia antiarrhythmic drug disopyramide.Cellular and molecular life sciences : CMLS · 2024Article
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- QTPhysiological reports · 2022Article
- Delayed Ventricular Repolarization and Sodium Channel Current Modification in a Mouse Model of Rett Syndrome.International journal of molecular sciences · 2022Article
- Effects of prolactin on ventricular myocyte shortening and calcium transport in the streptozotocin-induced diabetic rat.Heliyon · 2020Article
- The macrolide drug erythromycin does not protect the hERG channel from inhibition by thioridazine and terfenadine.Physiological reports · 2020Article
- Structural implications of hERG KThe Journal of biological chemistry · 2018Article
- Differential responses of rabbit ventricular and atrial transient outward current (IPhysiological reports · 2017Article
- Regional effects of streptozotocin-induced diabetes on shortening and calcium transport in epicardial and endocardial myocytes from rat left ventricle.Physiological reports · 2016Article
- Multiple ion channel block by the cation channel inhibitor SKF-96365 in myocytes from the rabbit atrioventricular node.Physiological reports · 2016Article
- Electrophysiological properties of myocytes isolated from the mouse atrioventricular node: L-type ICa, IKr, If, and Na-Ca exchange.Physiological reports · 2015Article
- Dapagliflozin reduces the amplitude of shortening and Ca(2+) transient in ventricular myocytes from streptozotocin-induced diabetic rats.Molecular and cellular biochemistry · 2015Article
- Suppression of the hERG potassium channel response to premature stimulation by reduction in extracellular potassium concentration.Physiological reports · 2014Article
- Ranolazine inhibition of hERG potassium channels: drug-pore interactions and reduced potency against inactivation mutants.Journal of molecular and cellular cardiology · 2014Article
- Acute desensitization of acetylcholine and endothelin-1 activated inward rectifier K+ current in myocytes from the cardiac atrioventricular node.Biochemical and biophysical research communications · 2012Article
- High potency inhibition of hERG potassium channels by the sodium-calcium exchange inhibitor KB-R7943.British journal of pharmacology · 2012Article
- Contractility of ventricular myocytes is well preserved despite altered mechanisms of Ca2+ transport and a changing pattern of mRNA in aged type 2 Zucker diabetic fatty rat heart.Molecular and cellular biochemistry · 2012Article
- Action potential clamp and pharmacology of the variant 1 Short QT Syndrome T618I hERG K⁺ channel.PloS one · 2012Article
- Modulation by endothelin-1 of spontaneous activity and membrane currents of atrioventricular node myocytes from the rabbit heart.PloS one · 2012Article
- β-Adrenoceptor/PKA-stimulation, Na(+)-Ca(2+) exchange and PKA-activated Cl(-) currents in rabbit cardiomyocytes: a conundrum.Cell calcium · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We have developed a technique for making a rapid solution change, whilst at the same time maintaining the temperature of the preparation at 37 degrees C. It is technically difficult to use rapid solution changes when experiments are performed at normal mammalian body temperature. As a solution is heated from room temperature to 37 degrees C, gas bubbles form in the rapid-flowing solution streams, and these disturb a cell or attached recording pipettes. We describe a system that has been developed to eliminate these problems. We show how to construct the different components of the system, and we have designed an electronic circuit to control solution changes. We have performed tests to characterise the function of this system. Solution flow out of the nozzle of the device (0.88 ml min-1, linear flow velocity 11.6 cm s-1) caused a fall in the steady-state temperature at the experimental preparation of only 0.3 degrees C. The device which takes between 0.5 and 1 s to completely change the superfusate of a single cell, was used routinely with five different experimental solutions. This system may be valuable in studies which require rapid solution changes to be performed at a normal mammalian body temperature.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.