Evidence map›Paper›PMID 8770594›Full record

ArticleGenetics1996

Germline transformation of Drosophila virilis mediated by the transposable element hobo.

E R Lozovskaya, D I Nurminsky, D L Hartl, D T Sullivan

Abstract read
In one paragraph

Article in Genetics, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Highly efficient integration and expression of piggyBac-derived cassettes in the honeybee (Apis mellifera).Proceedings of the National Academy of Sciences of the United States of America · 2014
    Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
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  8. Toward Anopheles transformation: Minos element activity in anopheline cells and embryos.Proceedings of the National Academy of Sciences of the United States of America · 2000
    Article
  9. Hyperactive transposase mutants of the Himar1 mariner transposon.Proceedings of the National Academy of Sciences of the United States of America · 1999
    Article
  10. In vivo transposition of mariner-based elements in enteric bacteria and mycobacteria.Proceedings of the National Academy of Sciences of the United States of America · 1999
    Article
  11. The lepidopteran transposon vector, piggyBac, mediates germ-line transformation in the Mediterranean fruit fly.Proceedings of the National Academy of Sciences of the United States of America · 1998
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

E R LozovskayaDepartment of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
D I Nurminsky
D L Hartl
D T Sullivan

Funding

NIGMS NIH HHS GM-33741
6 · The paper itself

Abstract

A laboratory strain of Drosophila virilis was genetically transformed with a hobo vector carrying the miniwhite cassette using a helper plasmid with an hsp70-driven hobo transposase-coding sequence. The rate of transformation was 0.5% per fertile GO animal. Three transgenic insertions were cloned and characterized and found to be authentic hobo insertions. These results, together with the known widespread distribution of hobo in diverse insect species, suggest that hobo and related transposable elements may be of considerable utility in the germline transformation of insects other than D. melanogaster.

Indexed as

DNA Transposable ElementsTransformation, GeneticAnimalsBase SequenceCloning, MolecularDNA NucleotidyltransferasesDNA PrimersDrosophilaFemaleGenetic VectorsHSP70 Heat-Shock ProteinsIn Situ HybridizationMaleMolecular Sequence DataPlasmidsRepetitive Sequences, Nucleic AcidDNA NucleotidyltransferasesDNA PrimersDNA Transposable ElementsHSP70 Heat-Shock ProteinsTransposases

Identifiers

PMID8770594
PMCPMC1206945

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.