ArticleBiophysical journal1996
Preparation of bead-tailed actin filaments: estimation of the torque produced by the sliding force in an in vitro motility assay.
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed.
- Mechanics of blunting of actin-myosin interaction dynamics by the actinopathy-causing mutation E334Q in cytoskeletal γ-actin.The Journal of physiology · 2026Article
- Force and kinetics of fast and slow muscle myosin determined with a synthetic sarcomere-like nanomachine.Communications biology · 2024Article
- Myosin and tropomyosin-troponin complementarily regulate thermal activation of muscles.The Journal of general physiology · 2023Article
- Exploitation of Engineered Light-Switchable Myosin XI for Nanotechnological Applications.ACS nano · 2023Article
- Molecular-level evidence of force maintenance by smooth muscle myosin during LC20 dephosphorylation.The Journal of general physiology · 2022Article
- Biased localization of actin binding proteins by actin filament conformation.Nature communications · 2020Article
- A Myosin II-Based Nanomachine Devised for the Study of CaInternational journal of molecular sciences · 2020Article
- Three-dimensional wing structure attenuates aerodynamic efficiency in flapping fly wings.Journal of the Royal Society, Interface · 2020Article
- Microscopic heat pulses activate cardiac thin filaments.The Journal of general physiology · 2019Article
- Functional significance of HCM mutants of tropomyosin, V95A and D175N, studied withBiophysics and physicobiology · 2019Article
- A myosin II nanomachine mimicking the striated muscle.Nature communications · 2018Article
- Article
- Quantitative analysis of the lamellarity of giant liposomes prepared by the inverted emulsion method.Biophysical journal · 2014Article
- Article
- A novel single virus infection system reveals that influenza virus preferentially infects cells in g1 phase.PloS one · 2013Article
- Translational actomyosin research: fundamental insights and applications hand in hand.Journal of muscle research and cell motility · 2012Review
- Fluorescence microscopy for simultaneous observation of 3D orientation and movement and its application to quantum rod-tagged myosin V.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The role of tropomyosin domains in cooperative activation of the actin-myosin interaction.Journal of molecular biology · 2011Article
- Twirling of actin by myosins II and V observed via polarized TIRF in a modified gliding assay.Biophysical journal · 2008Article
- Measuring molecular rupture forces between single actin filaments and actin-binding proteins.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
By coating covalently the surface of a polystyrene bead (diameter = 1 micron) with gelsolin, we have succeeded in attaching the bead selectively at the barbed end of an actin filament and forming a 1:1 bead-actin filament complex. On a layer of heavy meromyosin on a nitrocellulose-coated coverglass, this bead-actin filament complex slid smoothly, trailing the bead at its end. Therefore we called this preparation "bead-tailed" actin filaments. The sliding velocity was indistinguishable from that of nonbeaded filaments. With use of this system, we tried to detect the axial rotation (rotation around the filament axis) in a sliding actin filament. Although a single bead at the tail end did not serve as the marker for the axial rotation, we occasionally found another bead bound to the tail bead. In this case, the orientation of the bead-aggregate could be followed continuously with a video monitor while the filament was sliding over heavy meromyosin. We observed that actin filaments slid over distances of many tens of micrometers without showing a complete turn of the bead-aggregates. On the basis of the calculation of rotational friction drag on the bead-aggregate, we estimate that the rotational component of the sliding force and the torque produced on a sliding actin filament (length < or = 10 microns) did not accumulate > 1 pN and 5 pN.nm, respectively. In the present system of randomly oriented heavy meromyosin lying on a nitrocellulose film without an external load.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.