ArticleThe Plant cell1996
The major integral proteins of spinach leaf plasma membranes are putative aquaporins and are phosphorylated in response to Ca2+ and apoplastic water potential.
Article in The Plant cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
70 citing papers in PubMed, 301 citations in OpenAlex.
- Ectomycorrhizal fungi increase phosphorus uptake in Pinus sylvestris var. mongolica and the mechanism controlling Sphaeropsis sapinea.BMC plant biology · 2026Article
- Post-translational modification acts as a digital like switch influencing AtPIP2;1 water and cation permeability.Scientific reports · 2025Article
- Overexpression of theFrontiers in plant science · 2025Article
- Aquaporin Modulation by Cations, a Review.Current issues in molecular biology · 2024Review
- Functional regulation of aquaporin dynamics by lipid bilayer composition.Nature communications · 2024Article
- Functional Regulation of Aquaporin Dynamics by Lipid Bilayer Composition.bioRxiv : the preprint server for biology · 2023Article
- Cost-benefit analysis of mesophyll conductance: diversities of anatomical, biochemical and environmental determinants.Annals of botany · 2022Review
- Calcium-dependent protein kinase 16 phosphorylates and activates the aquaporin PIP2;2 to regulate reversible flower opening in Gentiana scabra.The Plant cell · 2022Article
- Aquaporin OsPIP2;2 links the H2O2 signal and a membrane-anchored transcription factor to promote plant defense.Plant physiology · 2022Article
- Hormonal and environmental signaling pathways target membrane water transport.Plant physiology · 2021Review
- Deciphering the Role of Ion Channels in Early Defense Signaling against Herbivorous Insects.Cells · 2021Review
- Salt Stress Signals on Demand: Cellular Events in the Right Context.International journal of molecular sciences · 2020Review
- Sweet Cherry (International journal of molecular sciences · 2020Article
- Molecular and Functional Characterization of Grapevine NIPs through Heterologous Expression inInternational journal of molecular sciences · 2020Article
- Oscillating Aquaporin Phosphorylation and 14-3-3 Proteins Mediate the Circadian Regulation of Leaf Hydraulics.The Plant cell · 2019Article
- Plant Aquaporins in Infection by and Immunity Against Pathogens - A Critical Review.Frontiers in plant science · 2019Article
- Article
- Review
- The Interactions of Aquaporins and Mineral Nutrients in Higher Plants.International journal of molecular sciences · 2016Review
- Human Aquaporin-4 and Molecular Modeling: Historical Perspective and View to the Future.International journal of molecular sciences · 2016Review
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We show that homologs of the major intrinsic protein (MIP) family are major integral proteins of the spinach leaf plasma membrane and constitute approximately 20% of integral plasma membrane protein. By using oligonucleotide primers based on partial amino acid sequences for polymerase chain reaction and screening of a spinach leaf cDNA library, we obtained two full-length clones of MIP homologs (pm28a and pm28b). One of these clones, pm28a, was sequenced, and it encodes a protein (PM28A) of 281 amino acids with a molecular mass of 29.9 kD. DNA gel blots indicated that PM28A is the product of a single gene, and RNA gel blots showed that pm28a is ubiquitously expressed in the plant. In vivo phosphorylation of the 28-kD polypeptide(s), corresponding to PM28A and PM28B, was dependent on apoplastic water potential, suggesting a role in regulation of cell turgor for these putative aquaporins. In vitro, only one of the homologs, PM28A, was phosphorylated. Phosphorylation of PM28A occurred on Ser-274, seven amino acids from the C terminus of the protein, within a consensus phosphorylation site (Ser-X-Arg) for vertebrate protein kinase C. In vitro phosphorylation of PM28A was due to a plasma membrane-associated protein kinase and was strictly dependent on submicromolar concentrations of Ca2+.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.