ArticlePflugers Archiv : European journal of physiology1996
Quantitative RT-PCR analysis of mRNAs encoding a colonic putative H, K-ATPase alpha subunit along the rat nephron: effect of K+ depletion.
Article in Pflugers Archiv : European journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 42 citations in OpenAlex.
- Renal KFrontiers in physiology · 2023Review
- Deletion of the serine protease CAP2/Tmprss4 leads to dysregulated renal water handling upon dietary potassium depletion.Scientific reports · 2019Article
- Parameter estimation for mathematical models of a nongastric H+(Na+)-K(+)(NH4+)-ATPase.American journal of physiology. Renal physiology · 2015Article
- Regulation of transport in the connecting tubule and cortical collecting duct.Comprehensive Physiology · 2012Review
- The renal H+-K+-ATPases: physiology, regulation, and structure.American journal of physiology. Renal physiology · 2010Review
- Regulation of potassium (K) handling in the renal collecting duct.Pflugers Archiv : European journal of physiology · 2009Review
- GDF15 triggers homeostatic proliferation of acid-secreting collecting duct cells.Journal of the American Society of Nephrology : JASN · 2008Article
- Molecular identification of Sch28080-sensitive K-ATPase activities in the mouse kidney.Pflugers Archiv : European journal of physiology · 2006Article
- Increased sensitivity to K+ deprivation in colonic H,K-ATPase-deficient mice.The Journal of clinical investigation · 1998Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rat nephron displays two ouabain-sensitive K-ATPases: one, which is present in proximal tubules and thick ascending limbs of normal rats, is specifically activated by K+ and is down-regulated by K+ depletion, whereas the other one appears in collecting ducts of K+-depleted rats and is activated by either Na+ or K+. To determine which of these two ATPases is similar to colonic-type H,K-ATPase, we quantitated by reverse transcriptase-polymerase chain reaction (RT-PCR) the mRNAs encoding the colonic H,K-ATPase alpha subunit in microdissected nephron segments. In normal rats, statistically significant amounts of colonic H,K-ATPase mRNAs were detected exclusively in cortical thick ascending limbs and cortical collecting ducts (200-500 copies/mm). Because these levels of expression were low (1-1.2 copies/target cell), they probably have no physiological relevance. In rats fed a K+-depleted diet for 2 weeks, expression of colonic H,K-ATPase was markedly enhanced in cortical and medullary collecting ducts (5000-12,000 copies/mm or 30-40 copies per cell), whereas it remained low in all other nephron segments. Thus, colonic H,K-ATPase alpha subunit is specifically expressed in cortical and outer medullary collecting ducts of K+-depleted rats where it likely accounts for the ouabain-sensitive K-ATPase activity.
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