ArticleJournal of virology1996
cis-active structural motifs involved in specific encapsidation of Moloney murine leukemia virus RNA.
Article in Journal of virology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication.FEMS microbiology reviews · 2026Review
- Review
- NMR Studies of Retroviral Genome Packaging.Viruses · 2020Review
- NXF1 and CRM1 nuclear export pathways orchestrate nuclear export, translation and packaging of murine leukaemia retrovirus unspliced RNA.RNA biology · 2020Article
- Retroviral RNA Dimerization: From Structure to Functions.Frontiers in microbiology · 2018Review
- Review
- Review
- MLV requires Tap/NXF1-dependent pathway to export its unspliced RNA to the cytoplasm and to express both spliced and unspliced RNAs.Retrovirology · 2014Article
- Mechanism of enhanced mechanical stability of a minimal RNA kissing complex elucidated by nonequilibrium molecular dynamics simulations.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- MoMuLV and HIV-1 nucleocapsid proteins have a common role in genomic RNA packaging but different in late reverse transcription.PloS one · 2012Article
- Structural dynamics of retroviral genome and the packaging.Frontiers in microbiology · 2011Article
- Structure of a conserved retroviral RNA packaging element by NMR spectroscopy and cryo-electron tomography.Journal of molecular biology · 2010Article
- Murine leukemia virus RNA dimerization is coupled to transcription and splicing processes.Retrovirology · 2010Article
- Characterization of a natural heterodimer between MLV genomic RNA and the SD' retroelement generated by alternative splicing.RNA (New York, N.Y.) · 2007Article
- HIV controls the selective packaging of genomic, spliced viral and cellular RNAs into virions through different mechanisms.Nucleic acids research · 2007Article
- mRNA molecules containing murine leukemia virus packaging signals are encapsidated as dimers.Journal of virology · 2004Article
- RNA sequences in the Moloney murine leukemia virus genome bound by the Gag precursor protein in the yeast three-hybrid system.Journal of virology · 2004Article
- Complementarity-directed RNA dimer-linkage promotes retroviral recombination in vivo.Nucleic acids research · 2004Article
- In vitro characterization of a base pairing interaction between the primer binding site and the minimal packaging signal of avian leukosis virus genomic RNA.Nucleic acids research · 2003Article
- A new retroelement constituted by a natural alternatively spliced RNA of murine replication-competent retroviruses.The EMBO journal · 2003Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We have analyzed the roles of RNA structural motifs located in the 5' part of the Moloney murine leukemia virus (M-MuLV) encapsidation domain (Psi region) with regard to their effects on viral replication. Four putative stem-loop structures between the 5' splice donor site and the gag initiation codon have been examined: stem structure A, corresponding to M-MuLV viral nucleotides 211 to 224; stem-loop B, nucleotides 278 to 303; stem-loop C, nucleotides 310 to 352; and stem-loop D, nucleotides 355 to 374. By measuring infectivities, encapsidation and splicing efficiencies, and endogenous reverse transcription levels of motif A, B, C, and D deletion mutants, we identified mutations which affect replication at the encapsidation step. In particular, deletion of all four motifs in a single mutant eliminated encapsidation of viral RNA, while deletion of individual elements moderately reduced the encapsidation efficiencies. Through analysis of different deletion combinations, we found that deletion of the first two motifs (A plus B) reduced both encapsidation and reverse transcription efficiencies, while deletion of the 3' motifs (C plus D) eliminated encapsidation. Interestingly, the C and D motifs both contain a GACG loop sequence and are highly conserved among murine type C retroviruses. Our results indicate that M-MuLV motifs C and D are necessary for efficient encapsidation, and the presence of at least one of these two stem-loops is crucial to encapsidation and virus replication.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.