ArticleBritish journal of pharmacology1996
Mechanotransduction through the endothelial cytoskeleton: mediation of flow- but not agonist-induced EDRF release.
Article in British journal of pharmacology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 75 citations in OpenAlex.
- Endothelial Nucleoporin93 (Nup93) Maintains Vascular Homeostasis via Sun1-Dependent Regulation of RhoA/ROCK Activity.Arteriosclerosis, thrombosis, and vascular biology · 2026Article
- High shear stress enhances endothelial permeability in the presence of the risk haplotype at 9p21.3.APL bioengineering · 2021Article
- Endothelial basement membrane laminin 511 is essential for shear stress response.The EMBO journal · 2017Article
- AMP-Activated Protein Kinase and Sirtuin 1 Coregulation of Cortactin Contributes to Endothelial Function.Arteriosclerosis, thrombosis, and vascular biology · 2016Article
- Oscillatory fluid flow influences primary cilia and microtubule mechanics.Cytoskeleton (Hoboken, N.J.) · 2014Article
- Cell senescence reduced the mechanotransduction sensitivity of porcine angular aqueous plexus cells to elevation of pressure.Investigative ophthalmology & visual science · 2014Article
- Loss of syndecan-1 induces a pro-inflammatory phenotype in endothelial cells with a dysregulated response to atheroprotective flow.The Journal of biological chemistry · 2014Article
- Vascular control in humans: focus on the coronary microcirculation.Basic research in cardiology · 2009Review
- Mechanotransduction in vascular physiology and atherogenesis.Nature reviews. Molecular cell biology · 2009Review
- Role of the cytoskeleton in flow (shear stress)-induced dilation and remodeling in resistance arteries.Medical & biological engineering & computing · 2008Article
- Endothelial cytoskeletal elements are critical for flow-mediated dilation in human coronary arterioles.Medical & biological engineering & computing · 2008Article
- How steroid hormones act on the endothelium--insights by atomic force microscopy.Pflugers Archiv : European journal of physiology · 2008Review
- Life history of eNOS: partners and pathways.Cardiovascular research · 2007Review
- Differential inhibition by hyperglycaemia of shear stress- but not acetylcholine-mediated dilatation in the iliac artery of the anaesthetized pig.The Journal of physiology · 2006Article
- Development affects in vitro vascular tone and calcium sensitivity in ovine cerebral arteries.The Journal of physiology · 2004Article
- Absence of dystrophin in mice reduces NO-dependent vascular function and vascular density: total recovery after a treatment with the aminoglycoside gentamicin.Arteriosclerosis, thrombosis, and vascular biology · 2004Article
- Flow (shear stress)-induced endothelium-dependent dilation is altered in mice lacking the gene encoding for dystrophin.Circulation · 2001Article
- Role of shear stress in nitric oxide-dependent modulation of renal angiotensin II vasoconstriction.British journal of pharmacology · 1999Article
- Release of vasoactive substances from endothelial cells by shear stress and purinergic mechanosensory transduction.Journal of anatomy · 1999Review
- Isometric contraction induces the Ca2+-independent activation of the endothelial nitric oxide synthase.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. We have used a cascade bioassay system and isolated arterial ring preparations to investigate the contribution of the endothelial microfilament and microtubule cytoskeleton to EDRF release evoked by time-averaged shear stress and by acetylcholine in rabbit abdominal aorta. 2. Cytochalasin B (1 microM) and phalloidin (100 nM) were used to depolymerize and stabilize, respectively, F-actin microfilaments. Colchicine (500 nM) was used to inhibit tubulin dimerization and thus disrupt the microtubule network. Experiments were performed before or 1 h after administration of agents to the donor perfusate or organ bath. 3. In cascade bioassay studies, time-averaged shear stress was manipulated with dextran (1-4% w/v, 80,000 MW), to increase perfusate viscosity. EDRF release induced by increased perfusate viscosity was significantly (P < 0.01) attenuated by cytochalasin B, phalloidin and colchicine. 4. Endothelium-dependent relaxations to acetylcholine (0.01-30 microM) in cascade bioassay and in isolated aortic ring preparations were unaffected by pretreatment with any of these agents both in terms of their EC50 and maximal responses. Endothelium-independent relaxations to sodium nitroprusside (0.001-10 microM) were similarly unaffected. 5. We conclude that the endothelial F-actin microfilament and microtubule networks are involved in the mechanotransduction pathway for flow-evoked EDRF release in rabbit abdominal aorta. However, these cytoskeletal elements appear to play no role in acetylcholine-induced EDRF release in this tissue.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.