ArticleNucleic acids research1996
Kinetic studies on depurination and detritylation of CPG-bound intermediates during oligonucleotide synthesis.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 95 citations in OpenAlex.
- Syrah: a pipeline to maximize spatial transcriptomics data output.G3 (Bethesda, Md.) · 2026Article
- Development and Validation of an Ion-Pair Reverse-Phase High-Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry Method for Determination of Purity of Nusinersen for Quality Control of Drug Substance or Drug Product.International journal of molecular sciences · 2026Article
- Efficiency of Digital Photolithographic Synthesis of Large, High-Quality DNA Libraries and Microarrays using a Guanine OCommunications chemistry · 2025Article
- From liquid-phase synthesis to chemical ligation: preparation of oligonucleotides and their backbone analogs in solution.Nucleic acids research · 2025Review
- Recent progress in DNA data storage based on high-throughput DNA synthesis.Biomedical engineering letters · 2024Review
- Spatially Selective Electrochemical Cleavage of a Polymerase-Nucleotide Conjugate.ACS synthetic biology · 2023Article
- DNA synthesis technologies to close the gene writing gap.Nature reviews. Chemistry · 2023Review
- Quantification of synthetic errors during chemical synthesis of DNA and its suppression by non-canonical nucleosides.Scientific reports · 2022Article
- Unexpected Complexity in the Products Arising from NaOH-, Heat-, Amine-, and Glycosylase-Induced Strand Cleavage at an Abasic Site in DNA.Chemical research in toxicology · 2022Article
- Products Generated by Amine-Catalyzed Strand Cleavage at Apurinic/Apyrimidinic Sites in DNA: New Insights from a Biomimetic Nucleoside Model System.Chemical research in toxicology · 2022Article
- Hardware, Software, and Wetware Codesign Environment for Synthetic Biology.Biodesign research · 2022Review
- Uncertainties in synthetic DNA-based data storage.Nucleic acids research · 2021Review
- Mini review: Enzyme-based DNA synthesis and selective retrieval for data storage.Computational and structural biotechnology journal · 2021Review
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing.The Journal of organic chemistry · 2021Article
- Scalable One-Pot-Liquid-Phase Oligonucleotide Synthesis for Model Network Hydrogels.Journal of the American Chemical Society · 2020Article
- Synthesis of Disaccharide Nucleosides Utilizing the Temporary Protection of the 2',3'-cis-Diol of Ribonucleosides by a Boronic Ester.Molecules (Basel, Switzerland) · 2017Article
- Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology.Cold Spring Harbor perspectives in biology · 2017Review
- Large-scale de novo DNA synthesis: technologies and applications.Nature methods · 2014Review
- Optimized light-directed synthesis of aptamer microarrays.Analytical chemistry · 2013Article
- Efficiency, error and yield in light-directed maskless synthesis of DNA microarrays.Journal of nanobiotechnology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fully protected CPG-immobilized monomer, dimer and trimer oligonucleotides were used to study depurination during the chemical synthesis of oligonucleotides. Disappearance of the oligonucleotide during acid exposure time relative to an internal thymidine standard not subject to depurination was monitored by reverse phase HPLC analysis. Depurination half-times obtained for dichloroacetic acid (DCA) and trichloroacetic acid (TCA) in methylene chloride were found to be 3% DCA >> 15% DCA > 3% TCA. In order to understand the implications of depurination during DNA synthesis, the detritylation kinetics of model compounds DMT-dG-pT dimer and DMT-[17mer] mixed-base sequence were also measured. These results improve our ability to properly balance the contradictory goals of obtaining maximum detritylation with minimum depurination in oligonucleotide synthesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.