ArticleNucleic acids research1996
Acid binding and detritylation during oligonucleotide synthesis.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed, 38 citations in OpenAlex.
- 2'-O-Acetal Levulinic Ester Ribonucleoside 3'-Phosphoramidites for the Solid-Phase Synthesis of Long RNA.Current protocols · 2026Article
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing.The Journal of organic chemistry · 2021Article
- A microfluidic oligonucleotide synthesizer.Nucleic acids research · 2010Article
- Synthesis of high-quality libraries of long (150mer) oligonucleotides by a novel depurination controlled process.Nucleic acids research · 2010Article
- Kinetic studies on depurination and detritylation of CPG-bound intermediates during oligonucleotide synthesis.Nucleic acids research · 1996Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Under the conditions normally used for detritylation in oligonucleotide synthesis, the haloacetic acid binds strongly to the oligonucleotide. Acetonitrile also forms a complex with the deblocking acid, in competition with the oligonucleotide, and drastically slows detritylation. Incomplete removal of acetonitrile during the deblock step may slow the kinetics enough to result in incomplete detritylation of the oligonucleotide. Acid binding to the growing oligonucleotide causes striking chromatographic effects in the presence of high oligonucleotide mass densities. In packed-bed column reactors, at low linear velocities, the acid binding almost completely depletes free acid from the deblocking solution. This results in an advancing zone within which the oligonucleotide is saturated with acid. Detritylation occurs mostly in a narrow band at the front of the advancing saturated zone. Increasing the DCA concentration in order to achieve quick saturation can give faster and more complete detritylation while minimizing the exposure time of the oligonucleotide to acid.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.