ArticleNucleic acids research1996
Characterization of a dominant negative mutant form of the HNF-4 orphan receptor.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 25 citations in OpenAlex.
- Conjunction of potential G-quadruplex and adjacent cis-elements in the 5' UTR of hepatocyte nuclear factor 4-alpha strongly inhibit protein expression.Scientific reports · 2017Article
- Cell type-specific target selection by combinatorial binding of Smad2/3 proteins and hepatocyte nuclear factor 4alpha in HepG2 cells.The Journal of biological chemistry · 2011Article
- Hepatocyte nuclear factor-4alpha induces transdifferentiation of hematopoietic cells into hepatocytes.The Journal of biological chemistry · 2010Article
- The Peroxisomal 3-keto-acyl-CoA thiolase B Gene Expression Is under the Dual Control of PPARα and HNF4α in the Liver.PPAR research · 2010Article
- Mapping of HNF4alpha target genes in intestinal epithelial cells.BMC gastroenterology · 2009Article
- The MODY1 gene for hepatocyte nuclear factor 4alpha and a feedback loop control COUP-TFII expression in pancreatic beta cells.Molecular and cellular biology · 2008Article
- Dual promoters control the cell-specific expression of the human cell death-inducing DFF45-like effector B gene.The Biochemical journal · 2006Article
- HNF4alpha reduces proliferation of kidney cells and affects genes deregulated in renal cell carcinoma.Oncogene · 2005Article
- A developmental transition in definitive erythropoiesis: erythropoietin expression is sequentially regulated by retinoic acid receptors and HNF4.Genes & development · 2001Article
- Naturally occurring mutations in the human HNF4alpha gene impair the function of the transcription factor to a varying degree.Nucleic acids research · 2000Article
- An array of binding sites for hepatocyte nuclear factor 4 of high and low affinities modulates the liver-specific enhancer for the human alpha1-microglobulin/bikunin precursor.The Biochemical journal · 1998Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The HNF-4 orphan receptor is a member of the nuclear receptor family of transcription factors and a major regulator of genes involved in carbohydrate and lipid metabolism. As an initial step in characterizing the role of HNF-4 in the regulation of metabolism, we have generated a dominant negative form of HNF-4 (DN-HNF-4) that contains a defective DNA-binding domain. In gel mobility shift assays, DN-HNF-4 did not bind an oligonucleotide probe representing an essential HNF-4 binding site, C3P contained in the human apo CIII promoter, but did prevent the binding of two recombinant isoforms, HNF-4alpha1 and HNF-4alpha2, as well as naturally-occurring HNF-4. DN-HNF-4 had no effect on the binding of PPARgamma-RXRalpha heterodimers to a PPAR response element. In transfected HepG2 cells, DN-HNF-4 dramatically reduced constitutive transcriptional activity of the human apo CIII promoter and abolished the positive transcriptional activity caused by plasmids expressing either isoform of HNF-4. These results indicate that DN-HNF-4 is a selective dominant negative mutant which forms defective heterodimers with wild-type HNF-4, thereby preventing DNA binding and subsequent transcriptional activation by HNF-4.
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