ArticleThe Biochemical journal1996
Lack of integrin alpha-chain endoproteolytic cleavage in furin-deficient human colon adenocarcinoma cells LoVo.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 61 citations in OpenAlex.
- Beyond Processing: Furin as a Central Hub in Viral Pathogenesis and Genetic Susceptibility.Biomolecules · 2025Review
- Review
- Short linear motif candidates in the cell entry system used by SARS-CoV-2 and their potential therapeutic implications.Science signaling · 2021Review
- α6-Integrin alternative splicing: distinct cytoplasmic variants in stem cell fate specification and niche interaction.Stem cell research & therapy · 2018Review
- Article
- Proprotein convertases play an important role in regulating PKGI endoproteolytic cleavage and nuclear transport.American journal of physiology. Lung cellular and molecular physiology · 2013Article
- Enhanced UV-induced skin carcinogenesis in transgenic mice overexpressing proprotein convertases.Neoplasia (New York, N.Y.) · 2013Article
- Transgenic overexpression of the proprotein convertase furin enhances skin tumor growth.Neoplasia (New York, N.Y.) · 2012Article
- A systematic study of site-specific GalNAc-type O-glycosylation modulating proprotein convertase processing.The Journal of biological chemistry · 2011Article
- Genetic analysis of fin development in zebrafish identifies furin and hemicentin1 as potential novel fraser syndrome disease genes.PLoS genetics · 2010Article
- Activation-coupled membrane-type 1 matrix metalloproteinase membrane trafficking.The Biochemical journal · 2007Article
- Phylogenetic analysis of Amphioxus genes of the proprotein convertase family, including aPC6C, a marker of epithelial fusions during embryology.International journal of biological sciences · 2006Article
- The cysteine-rich domain of the secreted proprotein convertases PC5A and PACE4 functions as a cell surface anchor and interacts with tissue inhibitors of metalloproteinases.Molecular biology of the cell · 2005Article
- Endo/exo-proteolysis in neoplastic progression and metastasis.Journal of molecular medicine (Berlin, Germany) · 2005Review
- Inhibition of proprotein convertases enhances cell migration and metastases development of human colon carcinoma cells in a rat model.The American journal of pathology · 2004Article
- Processing of alpha4 integrin by the proprotein convertases: histidine at position P6 regulates cleavage.The Biochemical journal · 2003Article
- Down-regulation of alpha v/beta 3 integrin via misrouting to lysosomes by overexpression of a beta 3Lamp1 fusion protein.The Biochemical journal · 2003Article
- Proprotein convertases in tumor progression and malignancy: novel targets in cancer therapy.The American journal of pathology · 2002Review
- Platelet integrin alphaIIbbeta3-ligand interactions: what can we learn from the structure?International journal of hematology · 2001Review
- Endoproteolytic processing of integrin pro-alpha subunits involves the redundant function of furin and proprotein convertase (PC) 5A, but not paired basic amino acid converting enzyme (PACE) 4, PC5B or PC7.The Biochemical journal · 2000Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present report the biosynthesis of the integrin alpha-chains endowed with constitutive endoproteolytic cleavage was evaluated in LoVo cells where furin, a subtilisin-like convertase involved in post-translational endoproteolytic processing, is not functional. It was found that cell-surface alpha 3, alpha 6 and alpha v subunits were not processed endoproteolytically into heavy and light chains as they were in HT29-D4 cells, a furin-competent cell line. Complete removal of N-linked oligosaccharides and pulse-chase experiments confirmed that the cleavage of the alpha 6 integrin subunit occurring 45 min after translation in HT29 cells did not take place in LoVo cells. Apart from cleavage deficiency, alpha 6 subunit glycosylation, association with beta 4 subunits and targeting to the plasma membrane seemed comparable in LoVo and HT29 cells. The pro-alpha 6 and the pro-alpha 3 subunits immunopurified from LoVo cells were highly sensitive to endoproteolysis by recombinant furin. Furin cleavage was calcium dependent and resulted in the conversion of the 140 kDa pro-alpha 6 into a 120 kDa heavy chain. These results suggest strongly that furin is involved in the endoproteolytic processing of cleavable integrin alpha subunits.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.