ArticleThe Biochemical journal1996
Cytokeratin 8 released by breast carcinoma cells in vitro binds plasminogen and tissue-type plasminogen activator and promotes plasminogen activation.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 60 citations in OpenAlex.
- Neuronal conversion of single-chain tissue-type plasminogen activator into its two-chain form: implications in neurodevelopment, learning, and memory.Cell death & disease · 2025Article
- Group B Streptococcus Persistently Colonizing the Adenosquamous Carcinoma of the Lung: A Blessing in Disguise?Cureus · 2024Article
- Plasminogen Receptors and Fibrinolysis.International journal of molecular sciences · 2021Review
- Functions of the plasminogen receptor Plg-RJournal of thrombosis and haemostasis : JTH · 2020Review
- Externalized Keratin 8: A Target at the Interface of Microenvironment and Intracellular Signaling in Colorectal Cancer Cells.Cancers · 2018Article
- Epsilon-aminocaproic acid prevents high glucose and insulin induced-invasiveness in MDA-MB-231 breast cancer cells, modulating the plasminogen activator system.Molecular and cellular biochemistry · 2018Article
- Plasminogen-receptorJournal of thrombosis and haemostasis : JTH · 2017Article
- Cytokeratin 8 in Association with sdLDL and ELISA Development.North American journal of medical sciences · 2015Article
- New Insight on the Role of Plasminogen Receptor in Cancer Progression.Cancer growth and metastasis · 2015Review
- New insights into the role of Plg-RKT in macrophage recruitment.International review of cell and molecular biology · 2014Review
- Plasminogen receptors: the first quarter century.Seminars in thrombosis and hemostasis · 2013Review
- Three are better than one: plasminogen receptors as cancer theranostic targets.Experimental hematology & oncology · 2013Article
- Prostate cancer cell surface-associated keratin 8 and its implications for enhanced plasmin activity.The protein journal · 2012Article
- The Role of miRNAs as Key Regulators in the Neoplastic Microenvironment.Molecular biology international · 2011Article
- microRNA 17/20 inhibits cellular invasion and tumor metastasis in breast cancer by heterotypic signaling.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Overexpression of cell surface cytokeratin 8 in multidrug-resistant MCF-7/MX cells enhances cell adhesion to the extracellular matrix.Neoplasia (New York, N.Y.) · 2008Article
- Keratin 8 expression in head and neck epithelia.BMC cancer · 2008Article
- Widespread, exceptionally high levels of histone H3 lysine 4 trimethylation largely mediate "privileged" gene expression.Gene expression · 2007Article
- Analysis of differentially expressed proteins in cancerous and normal colonic tissues.World journal of gastroenterology · 2006Article
- Cleavage of host keratin 8 by a Chlamydia-secreted protease.Infection and immunity · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell-surface activation of plasminogen may be important in diseases that involve cellular migration, including atherosclerosis and tumour invasion/metastasis. Cytokeratin 8 (CK 8) has been identified as a plasminogen-binding protein expressed on the external surfaces of hepatocytes and breast carcinoma cells [Hembrough, Vasudevan, Allietta, Glass and Gonias (1995) J. Cell Sci. 108, 1071-1082]. In this investigation, we demonstrate that a soluble form of CK 8 is released into the culture medium of breast cancer cell lines. The released CK 8 is in the form of variably sized polymers that bind plasminogen and promote the activation of [Glu1]plasminogen and [Lys78]plasminogen by single-chain tissue-type plasminogen activator (sct-PA). To assess the mechanism by which CK 8 promotes plasminogen activation, CK 8 was purified from rat hepatocytes and immobilized in microtitre plates. Immobilized CK 8 bound 125I-plasminogen and 125I-sct-PA in a specific and saturable manner. The KDs were 160 +/- 40 nM and 250 +/- 48 nM, respectively. Activation of plasminogen bound to immobilized CK 8 was accelerated compared with plasminogen in solution, as determined using a coupled-substrate fluorescence assay and SDS/PAGE. The ability of CK 8 to promote plasminogen activation may be important in the pericellular spaces surrounding breast cancer cells and at the cell surface.
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