ArticleJournal of bacteriology1996
A study of mycobacterial transcriptional apparatus: identification of novel features in promoter elements.
Article in Journal of bacteriology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 149 citations in OpenAlex.
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- VapC proteins from Mycobacterium tuberculosis share ribonuclease sequence specificity but differ in regulation and toxicity.PloS one · 2018Article
- Promoter mutagenesis for fine-tuning expression of essential genes in Mycobacterium tuberculosis.Microbial biotechnology · 2018Article
- Functional differentiation of 3-ketosteroid ΔMicrobial cell factories · 2017Article
- REMap: Operon map of M. tuberculosis based on RNA sequence data.Tuberculosis (Edinburgh, Scotland) · 2016Article
- Mycobacterium tuberculosis Transcription Machinery: Ready To Respond to Host Attacks.Journal of bacteriology · 2016Review
- Regulation of the Alkane Hydroxylase CYP153 Gene in a Gram-Positive Alkane-Degrading Bacterium, Dietzia sp. Strain DQ12-45-1b.Applied and environmental microbiology · 2016Article
- Recombinant reporter assay using transcriptional machinery of Mycobacterium tuberculosis.Journal of bacteriology · 2015Article
- Mutational analysis of the mycobacteriophage BPs promoter PR reveals context-dependent sequences for mycobacterial gene expression.Journal of bacteriology · 2014Article
- The ftsZ Gene of Mycobacterium smegmatis is expressed Through Multiple Transcripts.The open microbiology journal · 2011Article
- Functional analysis of an intergenic non-coding sequence within mce1 operon of M.tuberculosis.BMC microbiology · 2010Article
- Mutational analysis of the -10 region from the Mycobacterium tuberculosis lipF promoter.Molecular biology reports · 2009Article
- RegAnalyst: a web interface for the analysis of regulatory motifs, networks and pathways.Nucleic acids research · 2009Article
- Delineating bacteriostatic and bactericidal targets in mycobacteria using IPTG inducible antisense expression.PloS one · 2009Article
- A novel gene, erm(41), confers inducible macrolide resistance to clinical isolates of Mycobacterium abscessus but is absent from Mycobacterium chelonae.Antimicrobial agents and chemotherapy · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Our earlier studies on transcriptional signals of mycobacteria had revealed that (i) strong promoters occur less frequently in the slowly growing pathogen Mycobacterium tuberculosis H37Rv than in the fast-growing saprophyte M. smegmatis and (ii) mycobacterial promoters function poorly in Escherichia coli. We now present evidence that RNA polymerases of M. smegmatis, M. tuberculosis, and M. bovis BCG recognize promoter elements with comparable efficiencies. Analysis of these randomly isolated mycobacterial promoters by DNA sequencing, primer extension, and deletion experiments revealed that their -10 regions are highly similar to those of E. coli promoters, in contrast to their -35 regions, which can tolerate a greater variety of sequences, owing presumably to the presence of multiple sigma factors with different or overlapping specificities for -35 regions, as reported earlier for the Streptomyces promoters. A comparison of the -10 and -35 binding domains of MysA, HrdB, and RpoD (the principal sigma factors of M. smegmatis, Streptomyces aureofaciens, and E. coli, respectively) showed that all three sigma factors have nearly identical -10 binding domains. However, the -35 binding domains of the principal mycobacterial and streptomycete sigma factors, although nearly identical to each other, are vastly different from the corresponding region of the sigma factor of E. coli. Thus, the transcriptional signals of mycobacteria have features in common with Streptomyces promoters but differ from those of E. coli because of major differences in the -35 regions of the promoters and the corresponding binding domain in the sigma factor.
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