ArticleNucleic acids research1996
Functional interaction between a RARE and an AP-2 binding site in the regulation of the human HOX A4 gene promoter.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 44 citations in OpenAlex.
- Altered epigenetic regulation of homeobox genes in human oral squamous cell carcinoma cells.Experimental cell research · 2014Article
- Genome-wide in silico identification of new conserved and functional retinoic acid receptor response elements (direct repeats separated by 5 bp).The Journal of biological chemistry · 2011Article
- CREG1 enhances p16(INK4a) -induced cellular senescence.Cell cycle (Georgetown, Tex.) · 2011Article
- Promoter hypermethylation silences expression of the HoxA4 gene and correlates with IgVh mutational status in CLL.Leukemia · 2006Article
- Article
- Persistence of gene expression changes in noninflamed and inflamed colonic mucosa in ulcerative colitis and their presence in colonic carcinoma.World journal of gastroenterology · 2005Article
- Evolutionary conservation of regulatory elements in vertebrate Hox gene clusters.Genome research · 2003Article
- A predictive model for regulatory sequences directing liver-specific transcription.Genome research · 2001Article
- Hox cluster genomics in the horn shark, Heterodontus francisci.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Dual role for transcription factor AP-2 in the regulation of the major fetal promoter P3 of the gene for human insulin-like growth factor II.The Biochemical journal · 1999Article
- Retardation of skeletal development and cervical abnormalities in transgenic mice expressing a dominant-negative retinoic acid receptor in chondrogenic cells.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
HOX A genes are induced in a temporal fashion after retinoic acid (RA) treatment in non-N-ras-transformed PA-1 human teratcarcinoma cells. However, In N-ras-transformed PA-1 cells, RA-Induced expression of HOX A genes is delayed. The mRNA for the transcriptional activator AP-2 is overexpressed in these ras-transformed cells, but AP-2 transcriptional activity is inhibited relative to non ras-transformed PA-1 cells. Constitutive expression of AP-2 mimics the effect of ras by transforming cells and inhibiting differentiation in culture. We analyzed 4 kb of the human HOX A4 gene promoter and identified seven putative AP-2-binding sites in the DNA sequence. Transcription assays with variably sized HOX A4 promoter reporter constructs revealed that a 365 bp region of the promoter, -2950 to -3315 relative to the mRNA start, controls RA responsiveness and ras-mediated inhibition of HOX A4 activity. This region contains an AP-2 binding site and a RARE. Elimination of the AP-2 site by site-directed mutagenesis demonstrated that the AP-2 site is involved in RA-mediated transcriptional activation of the human HOX A4 promoter in combination with the RA receptor response element (RARE). In N-ras-transformed cells, low HOX A4 promoter activity results from ras inhibition of AP-2 transactivation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.