ArticleMolecular and cellular biology1996
Protein-protein and protein-DNA interaction regions within the DNA end-binding protein Ku70-Ku86.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 111 citations in OpenAlex.
- DNA damage response and GATA4 signaling in cellular senescence and aging-related pathology.Frontiers in aging neuroscience · 2022Review
- Identification and functional characterization of a Ku-binding motif in aprataxin polynucleotide kinase/phosphatase-like factor (APLF).The Journal of biological chemistry · 2013Article
- CREB-binding protein regulates Ku70 acetylation in response to ionization radiation in neuroblastoma.Molecular cancer research : MCR · 2013Article
- Role of Ku70 and Bax in epigallocatechin-3-gallate-induced apoptosis of A549 cells in vivo.Oncology letters · 2013Article
- c-Myc suppression of DNA double-strand break repair.Neoplasia (New York, N.Y.) · 2012Article
- Computational studies on full-length Ku70 with DNA duplexes: base interactions and a helical path.Journal of molecular modeling · 2012Article
- Orphan receptor TR3 enhances p53 transactivation and represses DNA double-strand break repair in hepatoma cells under ionizing radiation.Molecular endocrinology (Baltimore, Md.) · 2011Article
- Host protein Ku70 binds and protects HIV-1 integrase from proteasomal degradation and is required for HIV replication.The Journal of biological chemistry · 2011Article
- Ku86 exists as both a full-length and a protease-sensitive natural variant in multiple myeloma cells.Cancer cell international · 2008Article
- 2-Step purification of the Ku DNA repair protein expressed in Escherichia coli.Protein expression and purification · 2007Article
- The life and death of DNA-PK.Oncogene · 2005Review
- DNA-dependent PK inhibits adeno-associated virus DNA integration.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Visualization of inositol phosphate-dependent mobility of Ku: depletion of the DNA-PK cofactor InsP6 inhibits Ku mobility.Nucleic acids research · 2004Article
- Poly(ADP-ribose) polymerase (PARP-1) is not involved in DNA double-strand break recovery.BMC cell biology · 2003Article
- The Gam protein of bacteriophage Mu is an orthologue of eukaryotic Ku.EMBO reports · 2003Article
- Specific interaction of IP6 with human Ku70/80, the DNA-binding subunit of DNA-PK.The EMBO journal · 2002Article
- In vivo association of Ku with mammalian origins of DNA replication.Molecular biology of the cell · 2001Article
- Prokaryotic homologs of the eukaryotic DNA-end-binding protein Ku, novel domains in the Ku protein and prediction of a prokaryotic double-strand break repair system.Genome research · 2001Article
- Inhibition of Ku heterodimer DNA end binding activity during granulocytic differentiation of human promyelocytic cell lines.Oncogene · 2001Article
- Human securin, hPTTG, is associated with Ku heterodimer, the regulatory subunit of the DNA-dependent protein kinase.Nucleic acids research · 2001Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA ends are generated during double-strand-break repair and recombination. A p70-p86 heterodimer, Ku, accounts for the DNA end binding activity in eukaryotic cell extracts. When one or both subunits of Ku are missing, mammalian cells are deficient in double-strand-break repair and in specialized recombination, such as V(D)J recombination. Little is known of which regions of Ku70 and Ku86 bind to each other to form the heterodimeric complex or of which regions are important for DNA end binding. We have done genetic and biochemical studies to examine the domains within the two subunits important for protein assembly and for DNA end binding. We found that the C-terminal 20-kDa region of Ku70 and the C-terminal 32-kDa region of Ku86 are important for subunit-subunit interaction. For DNA binding, full-length individual subunits are inactive, indicating that heterodimer assembly precedes DNA binding. DNA end binding activity by the heterodimer requires the C-terminal 40-kDa region of Ku70 and the C-terminal 45-kDa region of Ku86. Leucine zipper-like motifs in both subunits that have been suggested as the Ku70-Ku86 interaction domains do not appear to be the sites of such interaction because these are dispensable for both assembly and DNA end binding. On the basis of these studies, we have organized Ku70 into nine sequence regions conserved between Saccharomyces cerevisiae, Drosophila melanogaster, mice, and humans; only the C-terminal three regions are essential for assembly (amino acids [aa] 439 to 609), and the C-terminal four regions appear to be essential for DNA end binding (aa 254 to 609). Within the minimal active fragment of Ku86 necessary for subunit interaction (aa 449 to 732) and DNA binding (aa 334 to 732), a proline-rich region is the only defined motif.
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