ArticleMolecular and cellular biology1996
Sex-lethal interacts with splicing factors in vitro and in vivo.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- The mNature communications · 2017Article
- Promiscuity in post-transcriptional control of gene expression: Drosophila sex-lethal and its regulatory partnerships.FEBS letters · 2017Review
- The translation initiation factor eIF4E regulates the sex-specific expression of the master switch gene Sxl in Drosophila melanogaster.PLoS genetics · 2011Article
- The functioning of the Drosophila CPEB protein Orb is regulated by phosphorylation and requires casein kinase 2 activity.PloS one · 2011Article
- Article
- Glorund interactions in the regulation of gurken and oskar mRNAs.Developmental biology · 2009Article
- Functioning of the Drosophila Wilms'-tumor-1-associated protein homolog, Fl(2)d, in Sex-lethal-dependent alternative splicing.Genetics · 2008Article
- Sex-specific expression of alternative transcripts in Drosophila.Genome biology · 2006Article
- Article
- Article
- A co-repressor assembly nucleated by Sex-lethal in the 3'UTR mediates translational control of Drosophila msl-2 mRNA.The EMBO journal · 2003Article
- RNA-protein interactions that regulate pre-mRNA splicing.Gene expression · 2002Review
- GAGA factor isoforms have distinct but overlapping functions in vivo.Molecular and cellular biology · 2001Article
- Polypyrimidine tract binding protein antagonizes exon definition.Molecular and cellular biology · 2001Review
- Switch in 3' splice site recognition between exon definition and splicing catalysis is important for sex-lethal autoregulation.Molecular and cellular biology · 2001Article
- Functioning of the Drosophila integral U1/U2 protein Snf independent of U1 and U2 small nuclear ribonucleoprotein particles is revealed by snf(+) gene dose effects.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Combinatorial control of a neuron-specific exon.RNA (New York, N.Y.) · 1999Article
- An N-terminal truncation uncouples the sex-transforming and dosage compensation functions of sex-lethal.Molecular and cellular biology · 1999Article
- Trans-acting factors required for inclusion of regulated exons in the Ultrabithorax mRNAs of Drosophila melanogaster.Genetics · 1999Article
- Activities of the Sex-lethal protein in RNA binding and protein:protein interactions.Nucleic acids research · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Drosophila sex determination gene Sex-lethal controls its own expression and the expression of downstream target genes such as transformer by regulating RNA splicing. Genetic and molecular studies have established that Sxl requires the product of another gene, snf, to autoregulate the splicing of its own transcripts. snf has recently been shown to encode a Drosophila U1 and U2 small nuclear ribonucleoprotein particle protein. In the work reported here, we demonstrate that the Sxl and Snf proteins can interact directly in vitro and that these two proteins are part of an RNase-sensitive complex in vivo which can be immunoprecipitated with the Sxl antibody. Unlike bulk Snf protein, which sediments slowly in sucrose gradients, the Snf protein associated with Sxl is in a large, rapidly sedimenting complex. Detailed characterization of the Sxl-Snf complexes from cross-linked extracts indicates that these complexes contain additional small nuclear ribonucleoprotein particle proteins and the U1 and U2 small nuclear RNAs. Finally, consistent with the RNase sensitivity of the Sxl-Snf complexes, Sxl transcripts can also be immunoprecipitated by Sxl antibodies. On the basis of the physical interactions between Sxl and Snf, we present a model for Sxl splicing regulation. This model helps explain how the Sxl protein is able to promote the sex-specific splicing of Sxl transcripts, utilizing target sequences that are distant from the regulated splice sites.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.