ArticleMolecular and cellular biology1996
The POU domain of SCIP/Tst-1/Oct-6 is sufficient for activation of an acetylcholine receptor promoter.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Functional characterization of SNPs in CHRNA3/B4 intergenic region associated with drug behaviors.Brain research · 2013Article
- The nicotinic acetylcholine receptor CHRNA5/A3/B4 gene cluster: dual role in nicotine addiction and lung cancer.Progress in neurobiology · 2010Review
- Temporally- and spatially-regulated transcriptional activity of the nicotinic acetylcholine receptor beta4 subunit gene promoter.Neuroscience · 2010Article
- Shared long-range regulatory elements coordinate expression of a gene cluster encoding nicotinic receptor heteromeric subtypes.Molecular and cellular biology · 2006Article
- Identification of genes that are downregulated in the absence of the POU domain transcription factor pou3f1 (Oct-6, Tst-1, SCIP) in sciatic nerve.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- The ETS domain factor Pet-1 is an early and precise marker of central serotonin neurons and interacts with a conserved element in serotonergic genes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Cell type-specific activation of neuronal nicotinic acetylcholine receptor subunit genes by Sox10.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Accelerated nerve regeneration mediated by Schwann cells expressing a mutant form of the POU protein SCIP.The Journal of cell biology · 1998Article
- Evidence that the homeodomain protein Gtx is involved in the regulation of oligodendrocyte myelination.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- POU domain transcription factors in embryonic development.Molecular biology reports · 1997Review
- beta43': An enhancer displaying neural-restricted activity is located in the 3'-untranslated exon of the rat nicotinic acetylcholine receptor beta4 gene.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the PC12 neuroendocrine line, the neuronal nicotinic acetylcholine receptor alpha3 gene promoter is activated by SCIP/Tst-1/Oct-6, a POU domain transcription factor proposed to be important for regulating the development of specific neural cell populations. In this study, we have investigated the SCIP polypeptide domains involved in alpha3 promoter activation. The characteristics of activation by a chimeric effector in which the GAL4 DNA binding domain was substituted for the SCIP POU domain were dramatically different from those of wild-type SCIP. At low effector masses, the chimeric polypeptide weakly activated alpha3 in a GAL4 binding-site-dependent manner but then squelched transcription at higher masses. In contrast, wild-type SCIP activation was not modulated by the presence of multimerized SCIP binding sites, and squelching was not observed. Analysis of wild-type SCIP truncations revealed that deletion of the previously characterized SCIP amino-terminal activation domain did not destroy activity of the factor. Surprisingly, a truncation expressing nothing more than the POU domain was nearly as active as wild-type SCIP. Moreover, cotransfection of a GAL4-VP16 effector with an effector expressing just the SCIP POU domain resulted in synergistic activation of the promoter. Synergistic activation did not depend on an Sp1 motif that is the only functional alpha3 cis element outside the transcription start site region. Our results show that the DNA binding domain of a POU factor is capable of transcriptional activation probably through protein-protein interactions with components of the basal transcription complex.
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