ArticleMolecular and cellular biology1996
RNA structure is a critical determinant of poly(A) site recognition by cleavage and polyadenylation specificity factor.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 58 citations in OpenAlex.
- Fateful Decisions of Where to Cut the Line: Pathology Associated with Aberrant 3' End Processing and Transcription Termination.Journal of molecular biology · 2025Review
- Decoding biology with massively parallel reporter assays and machine learning.Genes & development · 2024Review
- Modulation of diverse biological processes by CPSF, the master regulator of mRNA 3' ends.RNA (New York, N.Y.) · 2024Review
- RNA architecture influences plant biology.Journal of experimental botany · 2021Review
- Conserved long-range base pairings are associated with pre-mRNA processing of human genes.Nature communications · 2021Article
- Advances in the Bioinformatics Knowledge of mRNA Polyadenylation in Baculovirus Genes.Viruses · 2020Review
- In cell mutational interference mapping experiment (in cell MIME) identifies the 5' polyadenylation signal as a dual regulator of HIV-1 genomic RNA production and packaging.Nucleic acids research · 2018Article
- Global mapping of DNA conformational flexibility on Saccharomyces cerevisiae.PLoS computational biology · 2015Article
- mRNA 3'end processing: A tale of the tail reaches the clinic.EMBO molecular medicine · 2014Review
- Putting an 'End' to HIV mRNAs: capping and polyadenylation as potential therapeutic targets.AIDS research and therapy · 2013Article
- Characterization of the human ornithine transcarbamylase 3' untranslated regulatory region.DNA and cell biology · 2012Article
- Structural basis of UGUA recognition by the Nudix protein CFI(m)25 and implications for a regulatory role in mRNA 3' processing.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- A physical and functional link between splicing factors promotes pre-mRNA 3' end processing.Nucleic acids research · 2009Article
- 3' end mRNA processing: molecular mechanisms and implications for health and disease.The EMBO journal · 2008Review
- Splicing factors stimulate polyadenylation via USEs at non-canonical 3' end formation signals.The EMBO journal · 2007Article
- Bioinformatic identification of candidate cis-regulatory elements involved in human mRNA polyadenylation.RNA (New York, N.Y.) · 2005Article
- Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase.The EMBO journal · 2004Article
- Downstream sequence elements with different affinities for the hnRNP H/H' protein influence the processing efficiency of mammalian polyadenylation signals.Nucleic acids research · 2002Article
- Assembly of the cleavage and polyadenylation apparatus requires about 10 seconds in vivo and is faster for strong than for weak poly(A) sites.Molecular and cellular biology · 1999Article
- Formation of mRNA 3' ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis.Microbiology and molecular biology reviews : MMBR · 1999Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Sequence conservation among mammalian poly(A) sites is limited to the sequence AAUAAA, coupled with an amorphous downstream U- or GU-rich region. Since these sequences may also occur within the coding region of mRNAs, additional information must be required to define authentic poly(A) sites. Several poly(A) sites have been shown to contain sequences outside the core elements that enhance the efficiency of 3' processing in vivo and in vitro. The human immunodeficiency virus type 1, equine infectious anemia virus, and adenovirus L1 3' processing enhancers have been shown to promote the binding of cleavage and polyadenylation specificity factor (CPSF), the factor responsible for recognition of AAUAAA, to the pre-mRNA, thereby facilitating the assembly of a stable 3' processing complex. We have used in vitro selection to examine the mechanism by which the human immunodeficiency virus type 1 3' processing enhancer promotes the interaction of CPSF with the AAUAAA hexamer. Surprisingly, RNAs selected for efficient polyadenylation were related by structure rather than sequence. Therefore, in the absence of extensive sequence conservation, our results strongly suggest that RNA structure is a critical determinant of poly(A) site recognition by CPSF and may play a key role in poly(A) site definition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.