ArticleMolecular and cellular biology1996
Composite patterns in neutral/neutral two-dimensional gels demonstrate inefficient replication origin usage.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- A binary self-amplifying expression platform enabling lipid nanoparticle-free vaccines and nanomedicines.Nature communications · 2025Article
- 2D gel electrophoresis reveals dynamics of t-loop formation during the cell cycle and t-loop in maintenance regulated by heterochromatin state.The Journal of biological chemistry · 2019Article
- Bubble-seq analysis of the human genome reveals distinct chromatin-mediated mechanisms for regulating early- and late-firing origins.Genome research · 2013Article
- Friedreich's ataxia-associated GAA repeats induce replication-fork reversal and unusual molecular junctions.Nature structural & molecular biology · 2013Article
- Location, location, location: it's all in the timing for replication origins.Genes & development · 2013Review
- Bubble-chip analysis of human origin distributions demonstrates on a genomic scale significant clustering into zones and significant association with transcription.Genome research · 2011Article
- Article
- Checkpoint-dependent regulation of origin firing and replication fork movement in response to DNA damage in fission yeast.Molecular and cellular biology · 2009Article
- Replication of vertebrate mitochondrial DNA entails transient ribonucleotide incorporation throughout the lagging strand.The EMBO journal · 2006Article
- The Chinese hamster dihydrofolate reductase replication origin decision point follows activation of transcription and suppresses initiation of replication within transcription units.Molecular and cellular biology · 2006Article
- Swi1 prevents replication fork collapse and controls checkpoint kinase Cds1.Molecular and cellular biology · 2003Article
- Spatial distribution and specification of mammalian replication origins during G1 phase.The Journal of cell biology · 2003Article
- Initiation sites are distributed at frequent intervals in the Chinese hamster dihydrofolate reductase origin of replication but are used with very different efficiencies.Molecular and cellular biology · 2002Article
- Article
- Lagging-strand, early-labelling, and two-dimensional gel assays suggest multiple potential initiation sites in the Chinese hamster dihydrofolate reductase origin.Molecular and cellular biology · 1998Article
- Characterizing replication intermediates in the amplified CHO dihydrofolate reductase domain by two novel gel electrophoretic techniques.Molecular and cellular biology · 1996Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The neutral/neutral two-dimensional (2-D) gel replicon mapping technique has been used to great advantage to localize and characterize origins of replication. Interestingly, many yeast origins display a composite pattern consisting of both a bubble arc and a single-fork arc. Moreover, in every instance in which neutral/neutral 2-D gels have been used to analyze origins in higher eukaryotic cells, two or more adjacent fragments display these composite patterns. We believe that composite patterns signal inefficient origin usage in yeast cells because the replicators in question are not active in every cell cycle and in higher eukaryotic replicons because initiation sites are chosen from among many potential sites lying within a zone. However, others have suggested that the single-fork arcs in these composite gel patterns arise from nicking activity that converts replication bubbles to branched structures that comigrate with bona fide single forks. Here, we have used three different replicon mapping strategies to show that broken simian virus 40 replication bubbles trace unique arcs that are clearly distinguishable from classic, intact single forks. Thus, it is likely that composite 2-D gel patterns represent origins that are inefficiently utilized.
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