ArticleMolecular and cellular biology1996
Phosphorylation of Ga14p at a single C-terminal residue is necessary for galactose-inducible transcription.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 53 citations in OpenAlex.
- Significant roles in RNA-binding for the amino-terminal regions of Drosophila Pumilio and Nanos.PLoS genetics · 2025Article
- Article
- TORC1 signaling modulates Cdk8-dependent GAL gene expression in Saccharomyces cerevisiae.Genetics · 2021Article
- Genome-wide expression and location analyses of the Candida albicans Tac1p regulon.Eukaryotic cell · 2007Article
- A fungal family of transcriptional regulators: the zinc cluster proteins.Microbiology and molecular biology reviews : MMBR · 2006Review
- Yeast Gal4: a transcriptional paradigm revisited.EMBO reports · 2006Review
- Article
- War1p, a novel transcription factor controlling weak acid stress response in yeast.Molecular and cellular biology · 2003Article
- Interaction of the Srb10 kinase with Sip4, a transcriptional activator of gluconeogenic genes in Saccharomyces cerevisiae.Molecular and cellular biology · 2001Article
- Multiple signals regulate GAL transcription in yeast.Molecular and cellular biology · 2000Article
- The regulator of the yeast proline utilization pathway is differentially phosphorylated in response to the quality of the nitrogen source.Molecular and cellular biology · 2000Article
- The Gal3p-Gal80p-Gal4p transcription switch of yeast: Gal3p destabilizes the Gal80p-Gal4p complex in response to galactose and ATP.Molecular and cellular biology · 1999Article
- Yeast carbon catabolite repression.Microbiology and molecular biology reviews : MMBR · 1998Review
- Functional relationships of Srb10-Srb11 kinase, carboxy-terminal domain kinase CTDK-I, and transcriptional corepressor Ssn6-Tup1.Molecular and cellular biology · 1998Article
- Identification of two independent transcriptional activation domains in the Autographa californica multicapsid nuclear polyhedrosis virus IE1 protein.Journal of virology · 1997Article
- Glucose derepression of gluconeogenic enzymes in Saccharomyces cerevisiae correlates with phosphorylation of the gene activator Cat8p.Molecular and cellular biology · 1997Article
- The DNA binding and activation domains of Gal4p are sufficient for conveying its regulatory signals.Molecular and cellular biology · 1997Article
- Galactose-dependent reversible interaction of Gal3p with Gal80p in the induction pathway of Gal4p-activated genes of Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- The Cdk8 kinase module regulates interaction of the mediator complex with RNA polymerase II.The Journal of biological chemistryArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gal4p regulates expression of genes necessary for galactose catabolism in Saccharomyces cerevisiae. We have previously shown that phosphorylation of Gal4p requires both its DNA binding and transcriptional-activation functions and have suggested that phosphorylation occurs as a consequence of interaction with general transcription factors. In this study, we show that phosphorylation occurs rapidly on a limited fraction of overexpressed Gal4p present in a sodium dodecyl sulfate-extractable subcellular fraction while a significant fraction remains stably unphosphorylated. Taken together with our previous observations, we conclude that Gal4p is phosphorylated only if it becomes localized to the nucleus and is capable of both DNA binding and transcriptional activation. We demonstrate that Gal4p is multiply phosphorylated at both the C and N termini, and we identify the precise locations of three sites of phosphorylation at serines 691, 696, and 699. Of these sites, only serine 699 must be phosphorylated for galactose-inducible transcription to occur. Mutation of S-699 to alanine significantly impairs GAL induction by galactose in GAL80+ cells but does not affect transcriptional activation by Gal4p in gal80- cells. In gal80- cells, Gal4p phosphorylation, including that of serine 699, is stimulated by the presence of both galactose and glucose, indicating that phosphorylation at this site is not specifically activated by galactose. Serine 699 phosphorylation requires Gal4p's DNA binding function and is influenced by the function of the RNA polymerase II holoenzyme component Gal11p. These results suggest that a phosphorylation on Gal4p, likely resulting from interaction with the holoenzyme, modulates the induction process by regulating interaction between Gal4p and Gal80p.
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