ArticleMolecular and cellular biology1996
Ca2+-calmodulin promotes survival of pheromone-induced growth arrest by activation of calcineurin and Ca2+-calmodulin-dependent protein kinase.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 104 citations in OpenAlex.
- Calcineurin promotes adaptation to chronic stress through two distinct mechanisms.Molecular biology of the cell · 2024Article
- Calcineurin promotes adaptation to chronic stress through two distinct mechanisms.bioRxiv : the preprint server for biology · 2024Article
- Cek1 regulates ß(1,3)-glucan exposure through calcineurin effectors in Candida albicans.PLoS genetics · 2022Article
- Comparative Transcriptome Analysis of Deep-Rooting and Shallow-Rooting Potato (Plants (Basel, Switzerland) · 2022Article
- Calmodulin kinase 2 genetically interacts with Rch1p to negatively regulate calcium import into Saccharomyces cerevisiae after extracellular calcium pulse.Archives of microbiology · 2022Article
- Coexistence of genetically different Rhizophagus irregularis isolates induces genes involved in a putative fungal mating response.The ISME journal · 2020Article
- The protein kinase Cmk2 negatively regulates the calcium/calcineurin signalling pathway and expression of calcium pump genes PMR1 and PMC1 in budding yeast.Cell communication and signaling : CCS · 2019Article
- Prolonged warm ischemia aggravates hepatic mitochondria damage and apoptosis in DCD liver by regulating CaInternational journal of clinical and experimental pathology · 2019Article
- Calcineurin Regulatory Subunit Calcium-Binding Domains Differentially Contribute to Calcineurin Signaling inGenetics · 2018Article
- Pheromone independent unisexual development in Cryptococcus neoformans.PLoS genetics · 2017Article
- Characterization of calcineurin from Cryptococcus humicola and the application of calcineurin in aluminum tolerance.BMC biotechnology · 2017Article
- Calcineurin, the CaMolecular biology of the cell · 2017Article
- The calcineruin inhibitor cyclosporine a synergistically enhances the susceptibility of Candida albicans biofilms to fluconazole by multiple mechanisms.BMC microbiology · 2016Article
- Kch1 family proteins mediate essential responses to endoplasmic reticulum stresses in the yeasts Saccharomyces cerevisiae and Candida albicans.The Journal of biological chemistry · 2013Article
- Regulation of cation balance in Saccharomyces cerevisiae.Genetics · 2013Review
- Activation of an essential calcium signaling pathway in Saccharomyces cerevisiae by Kch1 and Kch2, putative low-affinity potassium transporters.Eukaryotic cell · 2013Article
- 'Popping the clutch': novel mechanisms regulating sexual development in Cryptococcus neoformans.Mycopathologia · 2012Review
- Vacuolar H+-ATPase (V-ATPase) promotes vacuolar membrane permeabilization and nonapoptotic death in stressed yeast.The Journal of biological chemistry · 2012Article
- Regulation of cell wall biogenesis in Saccharomyces cerevisiae: the cell wall integrity signaling pathway.Genetics · 2011Review
- Localization and activity of the calcineurin catalytic and regulatory subunit complex at the septum is essential for hyphal elongation and proper septation in Aspergillus fumigatus.Molecular microbiology · 2011Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The cmd1-6 allele contains three mutations that block Ca2+ binding to calmodulin from Saccharomyces cerevisiae. We find that strains containing cmd1-6 lose viability during cell cycle arrest induced by the mating pheromone alpha-factor. The 50% lethal dose (LD50) of alpha-factor for the calmodulin mutant is almost fivefold below the LD50 for a wild-type strain. The calmodulin mutants are not more sensitive to alpha-factor, as measured by activation of a pheromone-responsive reporter gene. Two observations indicate that activation of the Ca2+-calmodulin-dependent protein phosphatase calcineurin contributes to survival of pheromone-induced arrest. First, deletion of the gene encoding the calcineurin regulatory B subunit, CNB1, from a wild-type strain decreases the LD50 of alpha-factor but has no further effect on a cmd1-6 strain. Second, a dominant constitutive calcineurin mutant partially restores the ability of the cmd1-6 strain to survive exposure to alpha-factor. Activation of the Ca2+-calmodulin-dependent protein kinase (CaMK) also contributes to survival, thus revealing a new function for this enzyme. Deletion of the CMK1 and CMK2 genes, which encode CaMK, decreases the LD50 of pheromone compared with that for a wild-type strain but again has no effect in a cmd1-6 strain. Furthermore, the LD50 of alpha-factor for a mutant in which the calcineurin and CaMK genes have been deleted is the same as that for the calmodulin mutant. Finally, the CaMK and calcineurin pathways appear to be independent since the ability of constitutive calcineurin to rescue a cmd1-6 strain is not blocked by deletion of the CaMK genes.
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