ArticleMolecular and cellular biology1996
The atrial natriuretic factor promoter is a downstream target for Nkx-2.5 in the myocardium.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
46 citing papers in PubMed, 154 citations in OpenAlex.
- NRSF/REST-Mediated Epigenomic Regulation in the Heart: Transcriptional Control of Natriuretic Peptides and Beyond.Biology · 2022Review
- BRD4 (Bromodomain-Containing Protein 4) Interacts with GATA4 (GATA Binding Protein 4) to Govern Mitochondrial Homeostasis in Adult Cardiomyocytes.Circulation · 2020Article
- Cardiac natriuretic peptides.Nature reviews. Cardiology · 2020Review
- The developing heart: fromDevelopment (Cambridge, England) · 2020Review
- Variation in a Left Ventricle-Specific Hand1 Enhancer Impairs GATA Transcription Factor Binding and Disrupts Conduction System Development and Function.Circulation research · 2019Article
- Article
- Wnt/β-Catenin Stimulation and Laminins Support Cardiovascular Cell Progenitor Expansion from Human Fetal Cardiac Mesenchymal Stromal Cells.Stem cell reports · 2016Article
- Article
- Signaling Pathways and Gene Regulatory Networks in Cardiomyocyte Differentiation.Tissue engineering. Part B, Reviews · 2015Review
- Article
- Lrrc10 is a novel cardiac-specific target gene of Nkx2-5 and GATA4.Journal of molecular and cellular cardiology · 2013Article
- Genetic and functional analysis of the NKX2-5 gene promoter in patients with ventricular septal defects.Pediatric cardiology · 2012Article
- Article
- Cardiac gene activation analysis in mammalian non-myoblasic cells by Nkx2-5, Tbx5, Gata4 and Myocd.PloS one · 2012Article
- Transactivation in Drosophila of human enhancers by human transcription factors involved in congenital heart diseases.Developmental dynamics : an official publication of the American Association of Anatomists · 2012Article
- Nkx2-5 represses Gata1 gene expression and modulates the cellular fate of cardiac progenitors during embryogenesis.Circulation · 2011Article
- Examining the cardiac NK-2 genes in early heart development.Pediatric cardiology · 2010Review
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- Nkx2-5 transactivates the Ets-related protein 71 gene and specifies an endothelial/endocardial fate in the developing embryo.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Cooperative interaction of Nkx2.5 and Mef2c transcription factors during heart development.Developmental dynamics : an official publication of the American Association of Anatomists · 2008Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The recently described NK2 family of homeodomain proteins are key developmental regulators. In Drosophila melanogaster, two members of this family, bagpipe and tinman, are required for visceral and cardiac mesoderm formation, respectively. In vertebrates, tinman appears to represent a family of closely related NK2 genes, including Nkx-2.5, that are expressed at an early stage in precardiac cells. Consistent with a role for Nkx-2.5 in heart development, inactivation of the Nkx-2.5 gene in mice causes severe cardiac malformations and embryonic lethality. However, little is known about the molecular action of Nkx-2.5 and its targets in cardiac muscle. In this paper, we report the identification and characterization of a functional and highly conserved Nkx-2.5 response element, termed the NKE, in the proximal region of the cardiac atrial natriuretic factor (ANF) promoter. The NKE is composed of two near-consensus NK2 binding sites that are each able to bind purified Nkx-2.5. The NKE is sufficient to confer cardiac cell-specific activity to a minimal TATA-containing promoter and is required for Nkx-2.5 activation of the ANF promoter in heterologous cells. Interestingly, in primary cardiocyte cultures, the NKE contributes to ANF promoter activity in a chamber- and developmental stage-specific manner, suggesting that Nkx-2.5 and/or other related cardiac proteins may play a role in chamber specification. This work provides the identification of a direct target for NK2 homeoproteins in the heart and lays the foundation for further molecular analyses of the role of Nkx-2.5 and other NK2 proteins in cardiac development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.