ArticleAmerican journal of human genetics1996
The retrieval of ancient human DNA sequences.
Article in American journal of human genetics, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
What it found
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Who cites it
68 citing papers in PubMed.
- Fifteenth century CE Bolivian maize reveals genetic affinities with ancient Peruvian maize.eLife · 2025Article
- AdDeam: a fast and scalable tool for estimating and clustering reference-level damage profiles.Bioinformatics (Oxford, England) · 2025Article
- Forensic Microbiology: When, Where and How.Microorganisms · 2024Review
- PCR in Forensic Science: A Critical Review.Genes · 2024Review
- Bioarchaeological insights into the last plague of Imola (1630-1632).Scientific reports · 2021Article
- A genomic and historical synthesis of plague in 18th century Eurasia.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Integrative approach usingProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Tuberculosis in post-contact Native Americans of Brazil: Paleopathological and paleogenetic evidence from the Tenetehara-Guajajara.PloS one · 2018Article
- Successful reconstruction of whole mitochondrial genomes from ancient Central America and Mexico.Scientific reports · 2017Article
- Human evolution: a tale from ancient genomes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- β-Thalassemia Distribution in the Old World: an Ancient Disease Seen from a Historical Standpoint.Mediterranean journal of hematology and infectious diseases · 2017Review
- Paleomicrobiology: a Snapshot of Ancient Microbes and Approaches to Forensic Microbiology.Microbiology spectrum · 2016Review
- Denisovans, Melanesians, Europeans, and Neandertals: The Confusion of DNA Assumptions and the Biological Species Concept.Journal of molecular evolution · 2016Article
- Re-inventing ancient human DNA.Investigative genetics · 2015Article
- Article
- Ancient DNA analysis of 8000 B.C. near eastern farmers supports an early neolithic pioneer maritime colonization of Mainland Europe through Cyprus and the Aegean Islands.PLoS genetics · 2014Article
- Separating endogenous ancient DNA from modern day contamination in a Siberian Neandertal.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- AmericaPlex26: a SNaPshot multiplex system for genotyping the main human mitochondrial founder lineages of the Americas.PloS one · 2014Article
- Preamplification procedure for the analysis of ancient DNA samples.TheScientificWorldJournal · 2013Article
- Ancient DNA studies: new perspectives on old samples.Genetics, selection, evolution : GSE · 2012Review
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA was extracted from approximately 600-year-old human remains found at an archaeological site in the southwestern United States, and mtDNA fragments were amplified by PCR. When these fragments were sequenced directly, multiple sequences seemed to be present. From three representative individuals, DNA fragments of different lengths were quantified and short overlapping amplification products cloned. When amplifications started from <40 molecules, clones contained several different sequences. In contrast, when they were initiated by a few thousand molecules, unambiguous and reproducible results were achieved. These results show that more experimental work than is often applied is necessary to ensure that DNA sequences amplified from ancient human remains are authentic. In particular, quantitation of the numbers of amplifiable molecules is a useful tool to determine the role of contaminating contemporary molecules and PCR errors in amplifications from ancient DNA.
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8755923PMC1914746What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.