ArticleProceedings of the National Academy of Sciences of the United States of America1996
Deletion of the mouse T-cell receptor beta gene enhancer blocks alphabeta T-cell development.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 176 citations in OpenAlex.
- Promoter- and enhancer-dependent cohesin loading initiates chromosome looping to fold Tcrb loci for long-range recombination.Cell reports · 2025Article
- Lineage- and stage-specific activity of antigen receptor gene enhancers during lymphocyte development.Frontiers in epigenetics and epigenomics · 2024Review
- Non-coding RNAs in immunoregulation and autoimmunity: Technological advances and critical limitations.Journal of autoimmunity · 2023Review
- Helix-Loop-Helix Proteins in Adaptive Immune Development.Frontiers in immunology · 2022Review
- Characterization of transcripts emanating from enhancer Eβ of the murine TCRβ locus.FEBS open bio · 2021Article
- Spatial Organization of Chromatin: Transcriptional Control of Adaptive Immune Cell Development.Frontiers in immunology · 2021Review
- Regulation of T-cell Receptor Gene Expression by Three-Dimensional Locus Conformation and Enhancer Function.International journal of molecular sciences · 2020Review
- Poor quality Vβ recombination signal sequences stochastically enforce TCRβ allelic exclusion.The Journal of experimental medicine · 2020Article
- Inefficient V(D)J recombination underlies monogenic T cell receptor β expression.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Two Successive Inversional Vβ Rearrangements on a SingleJournal of immunology (Baltimore, Md. : 1950) · 2020Article
- Ikaros prevents autoimmunity by controlling anergy and Toll-like receptor signaling in B cells.Nature immunology · 2019Article
- Precocious expression of Blimp1 in B cells causes autoimmune disease with increased self-reactive plasma cells.The EMBO journal · 2019Article
- A Lamina-Associated Domain Border Governs Nuclear Lamina Interactions, Transcription, and Recombination of the Tcrb Locus.Cell reports · 2018Article
- Identification of the transgenic integration site in 2C T cell receptor transgenic mice.Transgenic research · 2018Article
- Misregulation of theFrontiers in immunology · 2018Article
- Activation of MouseJournal of immunology (Baltimore, Md. : 1950) · 2017Article
- Access Guide to Antigen Receptor Genes.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- Distinct requirement of Runx complexes for TCRβ enhancer activation at distinct developmental stages.Scientific reports · 2017Article
- Identification of multi-loci hubs from 4C-seq demonstrates the functional importance of simultaneous interactions.Nucleic acids research · 2016Article
- Active and Inactive Enhancers Cooperate to Exert Localized and Long-Range Control of Gene Regulation.Cell reports · 2016Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intrathymic T-cell development requires temporally regulated rearrangement and expression of T-cell receptor (TCR) genes. To assess the role of the TCR beta gene transcriptional enhancer (Ebeta) in this process, mouse strains in which Ebeta is deleted were generated using homologous recombination techniques. We report that mice homozygous for the Ebeta deletion, whether a selectable marker gene is present or not, show a block in alphabeta T-cell development at the CD4-CD8- double-negative cell stage, whereas the number of gammadelta+ T cells is normal, few CD4+CD8+ double-positive thymocytes and no alphabeta+ T cells are produced. DNA-PCR and RNA-PCR analyses of thymic cells from homozygous mutants showed no evidence of TCR beta gene rearrangement although germ-line Vbeta transcripts were detected at a low level, in heterozygous T cells, the targeted allele is not rearranged. Thus, deletion of Ebeta totally prevents rearrangement, but not transcription, of the targeted beta locus. These data formally establish the critical role played by Ebeta in cis-activation of the TCR beta locus for V(D)J recombination during alphabeta T-cell development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.