ArticleProceedings of the National Academy of Sciences of the United States of America1996
Gene-targeted deletion and replacement mutations of the T-cell receptor beta-chain enhancer: the role of enhancer elements in controlling V(D)J recombination accessibility.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed.
- Promoter- and enhancer-dependent cohesin loading initiates chromosome looping to fold Tcrb loci for long-range recombination.Cell reports · 2025Article
- The insulator EACBE regulates V(D)J recombination of Tcrd gene by modulating chromatin organization.Frontiers in immunology · 2025Article
- Lineage- and stage-specific activity of antigen receptor gene enhancers during lymphocyte development.Frontiers in epigenetics and epigenomics · 2024Review
- Helix-Loop-Helix Proteins in Adaptive Immune Development.Frontiers in immunology · 2022Review
- Characterization of transcripts emanating from enhancer Eβ of the murine TCRβ locus.FEBS open bio · 2021Article
- Regulation of T-cell Receptor Gene Expression by Three-Dimensional Locus Conformation and Enhancer Function.International journal of molecular sciences · 2020Review
- Poor quality Vβ recombination signal sequences stochastically enforce TCRβ allelic exclusion.The Journal of experimental medicine · 2020Article
- Inefficient V(D)J recombination underlies monogenic T cell receptor β expression.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Two Successive Inversional Vβ Rearrangements on a SingleJournal of immunology (Baltimore, Md. : 1950) · 2020Article
- Misregulation of theFrontiers in immunology · 2018Article
- Distinct requirement of Runx complexes for TCRβ enhancer activation at distinct developmental stages.Scientific reports · 2017Article
- Identification of multi-loci hubs from 4C-seq demonstrates the functional importance of simultaneous interactions.Nucleic acids research · 2016Article
- Active and Inactive Enhancers Cooperate to Exert Localized and Long-Range Control of Gene Regulation.Cell reports · 2016Article
- Lineage-specific compaction of Tcrb requires a chromatin barrier to protect the function of a long-range tethering element.The Journal of experimental medicine · 2015Article
- Long-Range Regulation of V(D)J Recombination.Advances in immunology · 2015Review
- Epigenetic aspects of lymphocyte antigen receptor gene rearrangement or 'when stochasticity completes randomness'.Immunology · 2013Review
- An ectopic CTCF-dependent transcriptional insulator influences the choice of Vβ gene segments for VDJ recombination at TCRβ locus.Nucleic acids research · 2012Article
- Orchestrating T-cell receptor α gene assembly through changes in chromatin structure and organization.Immunologic research · 2011Article
- Recombination centres and the orchestration of V(D)J recombination.Nature reviews. Immunology · 2011Review
- A barrier-type insulator forms a boundary between active and inactive chromatin at the murine TCRβ locus.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
To assess the role of transcriptional enhancers in regulating accessibility of the T-cell receptor beta-chain (TCRbeta) locus, we generated embryonic stem cell lines in which a single allelic copy of the endogenous TCRbeta enhancer (Ebeta) was either deleted or replaced with the immunoglobulin heavy-chain intronic enhancer. We assayed the effects of these mutations on activation of the TCRbeta locus in normal T- and B-lineage cells by RAG-2 (recombination-activating gene 2)-deficient blastocyst complementation. We found that Ebeta is required for rearrangement and germ-line transcription of the TCRbeta locus in T-lineage cells. In the absence of Ebeta, the heavy-chain intronic enhancer partially supported joining region beta-chain rearrangement in T- but not in B-lineage cells. However, ability of the heavy-chain intronic enhancer to induce rearrangements was blocked by linkage to an expressed neomycin-resistance gene (neo(r)). These results demonstrate a critical role for Ebeta in promoting accessibility of the TCRbeta locus and suggest that additional negative elements may cooperate to further modulate this process.
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