ArticleProceedings of the National Academy of Sciences of the United States of America1996
Human fetal enterocytes in vitro: modulation of the phenotype by extracellular matrix.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
55 citing papers in PubMed, 130 citations in OpenAlex.
- Gastrointestinal tract, its pathophysiology andWorld journal of gastroenterology · 2025Review
- A developmental role for toll like receptor 4 in the neonatal intestine.Pediatric research · 2025Article
- Synergism between TLR4 and B. infantis in the development of the premature intestine.Pediatric research · 2025Article
- Culture media and format alter cellular composition and barrier integrity of porcine colonoid-derived monolayers.Tissue barriers · 2024Article
- Human milk microbiota: what did we learn in the last 20 years?Microbiome research reports · 2022Review
- The impact of indole-3-lactic acid on immature intestinal innate immunity and development: a transcriptomic analysis.Scientific reports · 2021Article
- Short-chain fatty acid butyrate, a breast milk metabolite, enhances immature intestinal barrier function genes in response to inflammation in vitro and in vivo.American journal of physiology. Gastrointestinal and liver physiology · 2021Article
- Preterm neonatal immunology at the intestinal interface.Cellular and molecular life sciences : CMLS · 2020Review
- Bioengineering NovelFrontiers in bioengineering and biotechnology · 2020Article
- Article
- The developmentally regulated fetal enterocyte gene,American journal of physiology. Gastrointestinal and liver physiology · 2019Article
- Development and biochemical and immunological characterization of early passage and immortalized bovine intestinal epithelial cell lines from the ileum of a young calf.Cytotechnology · 2019Article
- Development and characterization of 2-dimensional culture for buffalo intestinal cells.Cytotechnology · 2018Article
- Human Fetal-Derived Enterospheres Provide Insights on Intestinal Development and a Novel Model to Study Necrotizing Enterocolitis (NEC).Cellular and molecular gastroenterology and hepatology · 2018Article
- Human fetal intestinal epithelial cells metabolize and incorporate branched chain fatty acids in a structure specific manner.Prostaglandins, leukotrienes, and essential fatty acids · 2017Article
- 3D culture models of tissues under tension.Journal of cell science · 2017Review
- Article
- Diagnostic and Research Aspects of Small Intestinal Disaccharidases in Coeliac Disease.Journal of immunology research · 2017Review
- Article
- Anti-inflammatory effects of Bifidobacterium longum subsp infantis secretions on fetal human enterocytes are mediated by TLR-4 receptors.American journal of physiology. Gastrointestinal and liver physiology · 2016Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
The differentiation of small intestinal epithelial cells may require stimulation by microenvironmental factors in vivo. In this study, the effects of mesenchymal and luminal elements in nonmalignant epithelia] cells isolated from the human fetus were studied in vitro. Enterocytes from the human fetus were cultured and microenvironmental factors were added in stages, each stage more closely approximating the microenvironment in vivo. Four stages were examined: epithelial cells derived on plastic from intestinal culture and grown as a cell clone, the same cells grown on connective tissue support, primary epithelial explants grown on fibroblasts with a laminin base, and primary epithelial explants grown on fibroblasts and laminin with n-butyrate added to the incubation medium. The epithelial cell clone dedifferentiated when grown on plastic; however, the cells expressed cytokeratins and villin as evidence of their epithelial cell origin. Human connective tissue matrix from Engelbreth-Holm-Swarm sarcoma cells (Matrigel) modulated their phenotype: alkaline phosphatase activity increased, microvilli developed on their apical surface, and the profile of insulin-like growth factor binding proteins resembled that secreted by differentiated enterocytes. Epithelial cells taken directly from the human fetus as primary cultures and grown as explants on fibroblasts and laminin expressed greater specific enzyme activities in brush border membrane fractions than the cell clone. These activities were enhanced by the luminal molecule sodium butyrate. Thus the sequential addition of connective tissue and luminal molecules to nonmalignant epithelia] cells in vitro induces a spectrum of changes in the epithelial cell phenotype toward full differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.