ArticleMolecular and cellular biology1996
Precise alignment of sites required for mu enhancer activation in B cells.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- HMGA Proteins in Hematological Malignancies.Cancers · 2020Review
- Genome-Wide Identification of Target Genes for the Key B Cell Transcription FactorFrontiers in immunology · 2017Article
- Conservation and divergence of the Emicro3' enhancer in the IGH locus of teleosts.Immunogenetics · 2006Article
- Biased dA/dT somatic hypermutation as regulated by the heavy chain intronic iEmu enhancer and 3'Ealpha enhancers in human lymphoblastoid B cells.Molecular immunology · 2006Article
- A novel mammalian expression system derived from components coordinating nicotine degradation in arthrobacter nicotinovorans pAO1.Nucleic acids research · 2005Article
- Regulation of immunoglobulin gene transcription in a teleost fish: identification, expression and functional properties of E2A in the channel catfish.Immunogenetics · 2005Article
- Evolution of transcriptional enhancers in the immunoglobulin heavy-chain gene: functional characteristics of the zebrafish Emu3' enhancer.Immunogenetics · 2005Article
- Transcriptional repression of MMP-1 by p21SNFT and reduced in vitro invasiveness of hepatocarcinoma cells.Oncogene · 2004Article
- Precise arrangement of factor-binding sites is required for murine CD4 promoter function.Nucleic acids research · 2000Article
- Activation of c-myc promoter P1 by immunoglobulin kappa gene enhancers in Burkitt lymphoma: functional characterization of the intron enhancer motifs kappaB, E box 1 and E box 2, and of the 3' enhancer motif PU.Nucleic acids research · 2000Article
- Mutants of ETS domain PU.1 and GGAA/T recognition: free energies and kinetics.Protein science : a publication of the Protein Society · 1999Article
- Transcriptional activation by ETS and leucine zipper-containing basic helix-loop-helix proteins.Molecular and cellular biology · 1999Article
- Exploring functional redundancy in the immunoglobulin mu heavy-chain gene enhancer.Molecular and cellular biology · 1998Article
- ETS-mediated cooperation between basic helix-loop-helix motifs of the immunoglobulin mu heavy-chain gene enhancer.Molecular and cellular biology · 1998Article
- ETS-core binding factor: a common composite motif in antigen receptor gene enhancers.Molecular and cellular biology · 1998Article
- Combinatorial determinants of tissue-specific transcription in B cells and macrophages.Molecular and cellular biology · 1997Article
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Authors and funding
3 authors.
Funding
Abstract
The lymphocyte-specific immunoglobulin mu heavy-chain gene intronic enhancer is regulated by multiple nuclear factors. The previously defined minimal enhancer containing the muA, muE3, and muB sites is transactivated by a combination of the ETS-domain proteins PU.1 and Ets-1 in nonlymphoid cells. The core GGAAs of the muA and muB sites are separated by 30 nucleotides, suggesting that ETS proteins bind to these sites from these same side of the DNA helix. We tested the necessity for appropriate spatial alignment of these elements by using mutated enhancers with altered spacings. A 4- or 10-bp insertion between muE3 and muB inactivated the mu enhancer in S194 plasma cells but did not affect in vitro binding of Ets-1, PU.1, or the muE3-binding protein TFE3, alone or in pairwise combinations. Circular permutation and phasing analyses demonstrated that PU.1 binding but not TFE3 or Ets-1 bends mu enhancer DNA toward the major groove. We propose that the requirement for precise spacing of the muA and muB elements is due in part to a directed DNA bend induced by PU.1.
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