ArticleMolecular and cellular biology1996
Germ line-specific expression of intracisternal A-particle retrotransposons in transgenic mice.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 112 citations in OpenAlex.
- Co-evolving infectivity and expression patterns drive the diversification of endogenous retroviruses.The EMBO journal · 2026Article
- Loss of heterochromatin and retrotransposon silencing as determinants in oocyte aging.Aging cell · 2022Article
- Retrotransposons in the Mammalian Male Germline.Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation · 2022Review
- Mouse germ line mutations due to retrotransposon insertions.Mobile DNA · 2019Review
- rahu is a mutant allele of Dnmt3c, encoding a DNA methyltransferase homolog required for meiosis and transposon repression in the mouse male germline.PLoS genetics · 2017Article
- Exploratory bioinformatics investigation reveals importance of "junk" DNA in early embryo development.BMC genomics · 2017Article
- Locus-specific hypomethylation of the mouse IAP retrotransposon is associated with transcription factor-binding sites.Mobile DNA · 2017Article
- Genome-wide identification of endogenous RNA-directed DNA methylation loci associated with abundant 21-nucleotide siRNAs in Arabidopsis.Scientific reports · 2016Article
- Hide and seek: how chromatin-based pathways silence retroelements in the mammalian germline.Current opinion in genetics & development · 2016Review
- A Novel Terminal-Repeat Retrotransposon in Miniature (TRIM) Is Massively Expressed in Echinococcus multilocularis Stem Cells.Genome biology and evolution · 2015Article
- DNA methylation restrains transposons from adopting a chromatin signature permissive for meiotic recombination.Genes & development · 2015Article
- Embryonic expression of endogenous retroviral RNAs in somatic tissues adjacent to the Oikopleura germline.Nucleic acids research · 2015Article
- Setdb1 is required for germline development and silencing of H3K9me3-marked endogenous retroviruses in primordial germ cells.Genes & development · 2014Article
- Physiology of the read-write genome.The Journal of physiology · 2014Review
- Defending the genome from the enemy within: mechanisms of retrotransposon suppression in the mouse germline.Cellular and molecular life sciences : CMLS · 2014Review
- DNA methylation is dispensable for suppression of the agouti viable yellow controlling element in murine embryonic stem cells.PloS one · 2014Article
- Regulation of Mouse Retroelement MuERV-L/MERVL Expression by REX1 and Epigenetic Control of Stem Cell Potency.Frontiers in oncology · 2014Review
- Regulation of DNA transposition by CpG methylation and chromatin structure in human cells.Mobile DNA · 2013Article
- Is there a role for endogenous retroviruses to mediate long-term adaptive phenotypic response upon environmental inputs?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Intrinsic immunity against retrotransposons by APOBEC cytidine deaminases.Frontiers in microbiology · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intracisternal A-particle (IAP) sequences are endogenous retrovirus-like mobile elements, or retrotransposons, present at 1,000 copies in the mouse genome. These elements transpose in a replicative manner via an RNA intermediate and its reverse transcription, and their transposition should therefore be tightly controlled by their transcription level. To analyze the in vivo pattern of expression of these retrovirus-like elements, we constructed several independent transgenic mice with either a complete IAP element marked with an intron or with the IAP promoter, or long terminal repeat (LTR), alone controlling the expression of a lacZ reporter gene with a nuclear localization signal. For all transgenic lines analyzed, IAP expression as determined by reverse transcription-PCR analysis was found to be essentially restricted to the male germ line. Furthermore, in situ 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) staining of all organs disclosed specific beta-galactosidase-positive blue cells only within the testis, found as patches along the seminiferous tubules and often organized as assemblies of 2, 4, 8, or 16 cells. Histochemical analyses of tissues from 13.5-day-old embryos to adults demonstrated that this LTR activity is restricted to gonocytes and premeiotic undifferentiated spermatogonia. Finally, analysis of the methylation status of both transgenes and endogenous IAP LTRs demonstrated identical patterns, with methylation in somatic tissues and hypomethylation in the testis. Transgenic mice therefore reveal an intrinsic, highly restricted IAP expression which had escaped detection in previous global Northern (RNA) blot analyses and with possible strong biological relevance, as IAP activation specifically within the germ line might be a way to generate diversity at the evolutionary level without being deleterious to individuals.
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