ArticleMolecular and cellular biology1996
A consensus motif in the RFX DNA binding domain and binding domain mutants with altered specificity.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 73 citations in OpenAlex.
- An RFX transcription factor regulates ciliogenesis in the closest living relatives of animals.Current biology : CB · 2023Article
- Molecular characteristics and transcriptional regulatory of spermatogenesis-related geneAnimal reproduction · 2023Article
- The transcription factor Rfx7 limits metabolism of NK cells and promotes their maintenance and immunity.Nature immunology · 2018Article
- Characterization of the human RFX transcription factor family by regulatory and target gene analysis.BMC genomics · 2018Article
- RFX1 and RFX3 Transcription Factors Interact with the D Sequence of Adeno-Associated Virus Inverted Terminal Repeat and Regulate AAV Transduction.Scientific reports · 2018Article
- DNA-dependent formation of transcription factor pairs alters their binding specificity.Nature · 2015Article
- HEATR2 plays a conserved role in assembly of the ciliary motile apparatus.PLoS genetics · 2014Article
- Structure-aided prediction of mammalian transcription factor complexes in conserved non-coding elements.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Article
- Unbiased discovery of interactions at a control locus driving expression of the cancer-specific therapeutic and diagnostic target, mesothelin.Journal of proteome research · 2012Article
- oPOSSUM-3: advanced analysis of regulatory motif over-representation across genes or ChIP-Seq datasets.G3 (Bethesda, Md.) · 2012Article
- Forkhead transcription factor Fd3F cooperates with Rfx to regulate a gene expression program for mechanosensory cilia specialization.Developmental cell · 2012Article
- Fine tuning of RFX/DAF-19-regulated target gene expression through binding to multiple sites in Caenorhabditis elegans.Nucleic acids research · 2012Article
- The transcription factor Rfx3 regulates beta-cell differentiation, function, and glucokinase expression.Diabetes · 2010Article
- Multiplexed massively parallel SELEX for characterization of human transcription factor binding specificities.Genome research · 2010Article
- Article
- Distinct isoforms of the RFX transcription factor DAF-19 regulate ciliogenesis and maintenance of synaptic activity.Molecular biology of the cell · 2008Article
- Identification and characterization of novel human tissue-specific RFX transcription factors.BMC evolutionary biology · 2008Article
- Systematic discovery of regulatory motifs in conserved regions of the human genome, including thousands of CTCF insulator sites.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Autorepression of rfx1 gene expression: functional conservation from yeast to humans in response to DNA replication arrest.Molecular and cellular biology · 2005Article
- Characterization of dRFX2, a novel RFX family protein in Drosophila.Nucleic acids research · 2004Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The RFX DNA binding domain is a novel motif that has been conserved in a growing number of dimeric DNA-binding proteins, having diverse regulatory functions, in eukaryotic organisms ranging from yeasts to humans. To characterize this novel motif, we have performed a detailed dissection of the site-specific DNA binding activity of RFX1, a prototypical member of the RFX family. First, we have performed a site selection procedure to define the consensus binding site of RFX1. Second, we have developed a new mutagenesis-selection procedure to derive a precise consensus motif, and to test the accuracy of a secondary structure prediction, for the RFX domain. Third, a modification of this procedure has allowed us to isolate altered-specificity RFX1 mutants. These results should facilitate the identification both of additional candidate genes controlled by RFX1 and of new members of the RFX family. Moreover, the altered-specificity RFX1 mutants represent valuable tools that will permit the function of RFX1 to be analyzed in vivo without interference from the ubiquitously expressed endogenous protein. Finally, the simplicity, efficiency, and versatility of the selection procedure we have developed make it of general value for the determination of consensus motifs, and for the isolation of mutants exhibiting altered functional properties, for large protein domains involved in protein-DNA as well as protein-protein interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.