ArticleMolecular and cellular biology1996
A new class of activation-defective TATA-binding protein mutants: evidence for two steps of transcriptional activation in vivo.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 53 citations in OpenAlex.
- Spt3 and Spt8 Are Involved in the Formation of a Silencing Boundary by Interacting with TATA-Binding Protein.Biomolecules · 2023Article
- The Spt4-Spt5 complex: a multi-faceted regulator of transcription elongation.Biochimica et biophysica acta · 2013Review
- Transition step during assembly of HIV Tat:P-TEFb transcription complexes and transfer to TAR RNA.Molecular and cellular biology · 2012Article
- Article
- TATA-binding protein variants that bypass the requirement for Mot1 in vivo.The Journal of biological chemistry · 2009Article
- Saccharomyces cerevisiae HMO1 interacts with TFIID and participates in start site selection by RNA polymerase II.Nucleic acids research · 2008Article
- A TATA binding protein regulatory network that governs transcription complex assembly.Genome biology · 2007Article
- SAGA binds TBP via its Spt8 subunit in competition with DNA: implications for TBP recruitment.The EMBO journal · 2006Article
- The yeast FACT complex has a role in transcriptional initiation.Molecular and cellular biology · 2005Article
- Role for Nhp6, Gcn5, and the Swi/Snf complex in stimulating formation of the TATA-binding protein-TFIIA-DNA complex.Molecular and cellular biology · 2004Article
- TATA-binding protein mutants that are lethal in the absence of the Nhp6 high-mobility-group protein.Molecular and cellular biology · 2004Article
- Recruitment of Tup1-Ssn6 by yeast hypoxic genes and chromatin-independent exclusion of TATA binding protein.Eukaryotic cell · 2003Article
- The fission yeast TFIIB-related factor limits RNA polymerase III to a TATA-dependent pathway of TBP recruitment.Nucleic acids research · 2003Article
- Regulation of TATA-binding protein binding by the SAGA complex and the Nhp6 high-mobility group protein.Molecular and cellular biology · 2003Article
- Mutations in the histone fold domain of the TAF12 gene show synthetic lethality with the TAF1 gene lacking the TAF N-terminal domain (TAND) by different mechanisms from those in the SPT15 gene encoding the TATA box-binding protein (TBP).Nucleic acids research · 2003Article
- The TATA-binding protein is not an essential target of the transcriptional activators Gal4p and Gcn4p in Saccharomyces cerevisiae.The Biochemical journal · 2003Article
- SPN1, a conserved gene identified by suppression of a postrecruitment-defective yeast TATA-binding protein mutant.Genetics · 2002Article
- TATA-binding protein mutants that increase transcription from enhancerless and repressed promoters in vivo.Molecular and cellular biology · 2000Article
- Quantitation of RNA polymerase II and its transcription factors in an HeLa cell: little soluble holoenzyme but significant amounts of polymerases attached to the nuclear substructure.Molecular and cellular biology · 1999Article
- Synergistic transcriptional activation by TATA-binding protein and hTAFII28 requires specific amino acids of the hTAFII28 histone fold.Molecular and cellular biology · 1999Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Using a genetic screen, we isolated four TATA-binding protein (TBP) mutants that are specifically defective in vivo for the response to acidic activators. In contrast to previously described activation-defective TBP mutants, these TBP derivatives are not specifically defective for interactions with TATA elements or TFIIA. Three of these derivatives interact normally with a TATA element, TFIIA, TFIIB, or an acidic activation domain; presumably, they affect another protein-protein interaction important for transcriptional activation. The remaining derivative (with F-237 replaced by D) binds a TATA element with wild-type affinity, but the TBP-TATA complex has an altered electrophoretic mobility and interacts poorly with TFIIA and TFIIB; this suggests that the conformation of the TBP-TATA element complex plays a role in transcriptional activation. To determine the step at which the TBP derivatives were unable to activate transcription, we utilized an artificial recruitment assay in which TBP is targeted to the promoter via fusion to the LexA DNA-binding domain. Consistent with previous evidence that acidic activators can increase recruitment of TBP to the promoter in vivo, the activation defect of some of these TBP derivatives can be corrected by artificial recruitment. In contrast, the activation defect of the other TBP derivatives is not bypassed by artificial recruitment. Thus, these TBP mutants define two steps in the process of transcriptional stimulation by acidic activators: efficient recruitment to the TATA element and a postrecruitment interaction with a component(s) of the initiation complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.