ArticleMolecular and cellular biology1996
Mutations in two Ku homologs define a DNA end-joining repair pathway in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 122 papers.
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122 citing papers in PubMed, 272 citations in OpenAlex.
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- Telomeric amplicons ofbioRxiv : the preprint server for biology · 2026Article
- Unwrap RAP1's Mystery at Kinetoplastid Telomeres.Biomolecules · 2024Review
- Reconsidering pathway choice: a sequential model of mammalian DNA double-strand break pathway decisions.Current opinion in genetics & development · 2021Review
- Nuku, a family of primate retrocopies derived from KU70.G3 (Bethesda, Md.) · 2021Article
- The Multifaceted Roles of Ku70/80.International journal of molecular sciences · 2021Review
- Saccharomyces cerevisiae Mhr1 can bind Xho I-induced mitochondrial DNA double-strand breaks in vivo.Mitochondrion · 2018Article
- Ku DNA End-Binding Activity Promotes Repair Fidelity and Influences End-Processing During Nonhomologous End-Joining inGenetics · 2018Article
- Article
- An interplay between multiple sirtuins promotes completion of DNA replication in cells with short telomeres.PLoS genetics · 2018Article
- Efficient gene targeting in non-homologous end-joining-deficient Lipomyces starkeyi strains.Current genetics · 2017Article
- Inactivation of RAD52 and HDF1 DNA repair genes leads to premature chronological aging and cellular instability.Journal of biosciences · 2017Article
- Repair of UV-induced DNA lesions in natural Saccharomyces cerevisiae telomeres is moderated by Sir2 and Sir3, and inhibited by yKu-Sir4 interaction.Nucleic acids research · 2017Article
- Chromatin association of XRCC5/6 in the absence of DNA damage depends on the XPE gene product DDB2.Molecular biology of the cell · 2017Article
- The democratization of gene editing: Insights from site-specific cleavage and double-strand break repair.DNA repair · 2016Review
- The non-homologous end-joining pathway of S. cerevisiae works effectively in G1-phase cells, and religates cognate ends correctly and non-randomly.DNA repair · 2016Article
- Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance.Genes & development · 2016Article
- Requirement of POL3 and POL4 on non-homologous and microhomology-mediated end joining in rad50/xrs2 mutants of Saccharomyces cerevisiae.Mutagenesis · 2015Article
- Inhibition of telomere recombination by inactivation of KEOPS subunit Cgi121 promotes cell longevity.PLoS genetics · 2015Article
- RPA antagonizes microhomology-mediated repair of DNA double-strand breaks.Nature structural & molecular biology · 2014Article
62 more citing papers are in PubMed but not listed here.
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA double-strand break (DSB) repair in mammalian cells is dependent on the Ku DNA binding protein complex. However, the mechanism of Ku-mediated repair is not understood. We discovered a Saccharomyces cerevisiae gene (KU80) that is structurally similar to the 80-kDa mammalian Ku subunit. Ku8O associates with the product of the HDF1 gene, forming the major DNA end-binding complex of yeast cells. DNA end binding was absent in ku80delta, hdf1delta, or ku80delta hdf1delta strains. Antisera specific for epitope tags on Ku80 and Hdf1 were used in supershift and immunodepletion experiments to show that both proteins are directly involved in DNA end binding. In vivo, the efficiency of two DNA end-joining processes were reduced >10-fold in ku8Odelta, hdfldelta, or ku80delta hdf1delta strains: repair of linear plasmid DNA and repair of an HO endonuclease-induced chromosomal DSB. These DNA-joining defects correlated with DNA damage sensitivity, because ku80delta and hdf1delta strains were also sensitive to methylmethane sulfonate (MMS). Ku-dependent repair is distinct from homologous recombination, because deletion of KU80 and HDF1 increased the MMS sensitivity of rad52delta. Interestingly, rad5Odelta, also shown here to be defective in end joining, was epistatic with Ku mutations for MMS repair and end joining. Therefore, Ku and Rad50 participate in an end-joining pathway that is distinct from homologous recombinational repair. Yeast DNA end joining is functionally analogous to DSB repair and V(D)J recombination in mammalian cells.
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