ArticleMolecular and cellular biology1996
The SH3-domain protein Bem1 coordinates mitogen-activated protein kinase cascade activation with cell cycle control in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- A Protein-Protein Interaction Analysis Suggests a Wide Range of New Functions for the p21-Activated Kinase (PAK) Ste20.International journal of molecular sciences · 2023Article
- MoScd2 is involved in appressorium formation and pathogenicity via the Pmk1 MAPK pathway in Magnaporthe oryzae.Crop health · 2023Article
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- A time-resolved interaction analysis of Bem1 reconstructs the flow of Cdc42 during polar growth.Life science alliance · 2020Article
- Functions for Cdc42p BEM adaptors in regulating a differentiation-type MAP kinase pathway.Molecular biology of the cell · 2020Article
- Cellular and metabolic engineering of oleaginous yeastEngineering in life sciences · 2019Review
- Spatio-temporal MAPK dynamics mediate cell behavior coordination during fungal somatic cell fusion.Journal of cell science · 2018Article
- Mapping the Synthetic Dosage Lethality Network ofG3 (Bethesda, Md.) · 2017Article
- Predicting physiologically relevant SH3 domain mediated protein-protein interactions in yeast.Bioinformatics (Oxford, England) · 2016Article
- Mate and fuse: how yeast cells do it.Open biology · 2013Review
- Quantitative measurement of protein relocalization in live cells.Biophysical journal · 2013Article
- Conformational control of the Ste5 scaffold protein insulates against MAP kinase misactivation.Science (New York, N.Y.) · 2012Article
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- Symmetry breaking in the life cycle of the budding yeast.Cold Spring Harbor perspectives in biology · 2009Review
- Symmetry-breaking polarization driven by a Cdc42p GEF-PAK complex.Current biology : CB · 2008Article
- Central roles of small GTPases in the development of cell polarity in yeast and beyond.Microbiology and molecular biology reviews : MMBR · 2007Review
- Function of the MAPK scaffold protein, Ste5, requires a cryptic PH domain.Genes & development · 2006Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The mating mitogen-activated protein kinase (MAPK) cascade has three major outputs prior to fusion: transcriptional activation of many genes, cell cycle arrest in the G1 phase, and polarized growth. Bem1 localizes near the cortical actin cytoskeleton and is essential for polarized growth during mating. Here we show that Bem1 is required for efficient signal transduction and coordinates MAPK cascade activation with G1 arrest and mating. bem1delta null mutants are defective in G1 arrest and transcriptional activation in response to mating pheromone. Bem1 protein stimulates Fus3 (MAPK) activity and associates with Ste5, the tethering protein essential for activation of the MAPK kinase kinase Ste11. Bem1-Ste5 complexes also contain Ste11, Ste7 (MAPK kinase), and Fus3, suggesting that Ste5 localizes the MAPK cascade to Bem1. Strikingly, Bem1 also copurifies with Far1, a Fus3 substrate required for G1 arrest and proper polarized growth during mating. These and other results suggest that Bem1 may cross-link the Ste5-MAPK cascade complex to upstream activators and specific downstream substrates at the shmoo tip, thus enabling efficient circuitry for G1 arrest and mating.
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