ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1996
Multiple subtypes of voltage-gated calcium channel mediate transmitter release from parasympathetic neurons in the mouse bladder.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 72 citations in OpenAlex.
- Inhibition of Contractility of Isolated Caprine Detrusor by the Calcium Channel Blocker Cilnidipine and Reversal by Calcium Channel Openers.Current therapeutic research, clinical and experimental · 2023Article
- Selective reduction of neurotransmitter release by cAMP-dependent pathways in mouse detrusor.American journal of physiology. Regulatory, integrative and comparative physiology · 2022Article
- Frequency-dependent characteristics of nerve-mediated ATP and acetylcholine release from detrusor smooth muscle.Experimental physiology · 2022Article
- Electrophysiological properties of lumbosacral primary afferent neurons innervating urothelial and non-urothelial layers of mouse urinary bladder.Brain research · 2016Article
- 'Medusa head ataxia': the expanding spectrum of Purkinje cell antibodies in autoimmune cerebellar ataxia. Part 2: Anti-PKC-gamma, anti-GluR-delta2, anti-Ca/ARHGAP26 and anti-VGCC.Journal of neuroinflammation · 2015Review
- Spinal blockage of P/Q- or N-type voltage-gated calcium channels modulates functional and symptomatic changes related to haemorrhagic cystitis in mice.British journal of pharmacology · 2015Article
- Purinergic signalling in the urinary tract in health and disease.Purinergic signalling · 2014Review
- Article
- Modulation of purinergic neuromuscular transmission by phorbol dibutyrate is independent of protein kinase C in murine urinary bladder.The Journal of pharmacology and experimental therapeutics · 2012Article
- Prostaglandin E2 induces spontaneous rhythmic activity in mouse urinary bladder independently of efferent nerves.British journal of pharmacology · 2012Article
- John Newsom-Davis: clinician-scientist and so much more.Brain : a journal of neurology · 2011Article
- Calcium channel subtypes for cholinergic and nonadrenergic noncholinergic neurotransmission in isolated guinea pig trachea.Naunyn-Schmiedeberg's archives of pharmacology · 2010Article
- Antioxidant treatment restores prejunctional regulation of purinergic transmission in mesenteric arteries of deoxycorticosterone acetate-salt hypertensive rats.Neuroscience · 2010Article
- Spontaneous release of acetylcholine from autonomic nerves in the bladder.British journal of pharmacology · 2009Article
- N-type and P/Q-type calcium channels regulate differentially the release of noradrenaline, ATP and beta-NAD in blood vessels.Neuropharmacology · 2009Article
- Differential involvement of N-type calcium channels in transmitter release from vasoconstrictor and vasodilator neurons.British journal of pharmacology · 2004Article
- Ca2+ channels as targets of neurological disease: Lambert-Eaton Syndrome and other Ca2+ channelopathies.Journal of bioenergetics and biomembranes · 2003Review
- Differential facilitation of N- and P/Q-type calcium channels during trains of action potential-like waveforms.The Journal of physiology · 2002Article
- Lambert-Eaton myasthenic syndrome.Postgraduate medical journal · 1999Review
- Role of N-type calcium channels in autonomic neurotransmission in guinea-pig isolated left atria.British journal of pharmacology · 1999Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multiple subtypes of voltage-gated calcium channels are coupled to transmitter release from central neurons; however, only N-type channels have been shown to play a role in autonomic neurons. The aim of the present study was to investigate potential roles for other channel subtypes in transmitter release from parasympathetic neurons in the mouse bladder using calcium channel toxins alone and in combination. Transmitter release was measured indirectly by recording the contraction of bladder dome strips in response to electrical stimulation of the neurons by single pulses or trains of 20 pulses at 1-50 Hz. omega-Conotoxin-GVIA (GVIA) and omega-conotoxin-MVIIC (MVIIC) inhibited contractions in a concentration-dependent manner, with IC50 values of approximately 30 and 200 nM, respectively, at low stimulation frequencies. omega-Agatoxin-IVA (agatoxin) alone did not have any significant effect up to 300 nM. Cumulative addition of the toxins demonstrated that 300 nM agatoxin had a significant effect after N-type channels were blocked with 100 nM GVIA. MVIIC (3 microM) reduced the contraction amplitude further. Testing the toxins on the cholinergic or purinergic component of the contraction separately showed that acetylcholine release depends primarily on N-type channels and, to a lesser extent, on P- and Q-type channels, whereas ATP release involves predominantly P- and Q-type channels. In conclusion, parasympathetic neurons in the mouse bladder, like central neurons, use multiple calcium channel subtypes. Furthermore, the release of the two main transmitters in these neurons has differing dependencies on the calcium channel subtypes.
Indexed as
Identifiers
8753877PMC6579000W1873394831What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.