ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1996
Regulation of dendritic spine density in cultured rat hippocampal neurons by steroid hormones.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.
What it found
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The trial behind it
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Who cites it
101 citing papers in PubMed, 397 citations in OpenAlex.
- Relative effects of tamoxifen, raloxifene, and conjugated equine estrogens on cognition.Journal of women's health (2002) · 2010Trial
- Local synthesis and function of neuro-estrogen and neuro-androgen: impact on the hippocampus-related cognition and neuronal plasticity.Frontiers in cellular neuroscience · 2025Review
- The impact of estradiol on serotonin, glutamate, and dopamine systems.Frontiers in neuroscience · 2024Review
- Antidepressant-like effects of theFrontiers in pharmacology · 2023Article
- Behavioral and transcriptomic effects of the cancer treatment tamoxifen in mice.Frontiers in neuroscience · 2023Article
- Menstrual Cycle, Psychological Responses, and Adherence to Physical Exercise: Viewpoint of a Possible Barrier.Frontiers in psychology · 2021Article
- Dissecting Sex-Related Cognition between Alzheimer's Disease and Diabetes: From Molecular Mechanisms to Potential Therapeutic Strategies.Oxidative medicine and cellular longevity · 2021Review
- Rapid Estrogenic and Androgenic Neurosteroids Effects in the Induction of Long-Term Synaptic Changes: Implication for Early Memory Formation.Frontiers in neuroscience · 2020Review
- Sex-Specific Association of Lifetime Body Mass Index with Alzheimer's Disease Neuroimaging Biomarkers.Journal of Alzheimer's disease : JAD · 2020Article
- Chronic fluoxetine prevents fear memory generalization and enhances subsequent extinction by remodeling hippocampal dendritic spines and slowing down systems consolidation.Translational psychiatry · 2019Article
- Estradiol-Mediated Axogenesis of Hypothalamic Neurons Requires ERK1/2 and Ryanodine Receptors-Dependent Intracellular CaFrontiers in cellular neuroscience · 2019Article
- Assay of Calcium Transients and Synapses in Rat Hippocampal Neurons by Kinetic Image Cytometry and High-Content Analysis: An In Vitro Model System for Postchemotherapy Cognitive Impairment.Assay and drug development technologies · 2017Article
- Sex steroid hormones matter for learning and memory: estrogenic regulation of hippocampal function in male and female rodents.Learning & memory (Cold Spring Harbor, N.Y.) · 2015Review
- Utilizing induced pluripotent stem cells (iPSCs) to understand the actions of estrogens in human neurons.Hormones and behavior · 2015Review
- Molecular mechanisms underlying the memory-enhancing effects of estradiol.Hormones and behavior · 2015Review
- Selective estrogen receptor modulators (SERMs) enhance neurogenesis and spine density following focal cerebral ischemia.The Journal of steroid biochemistry and molecular biology · 2015Article
- Endogenous 17β-estradiol is required for activity-dependent long-term potentiation in the striatum: interaction with the dopaminergic system.Frontiers in cellular neuroscience · 2015Article
- Both GPER and membrane oestrogen receptor-α activation protect ventricular remodelling in 17β oestradiol-treated ovariectomized infarcted rats.Journal of cellular and molecular medicine · 2014Article
- Differential effects of synthetic progestagens on neuron survival and estrogen neuroprotection in cultured neurons.Molecular and cellular endocrinology · 2014Article
- Synaptodendritic recovery following HIV Tat exposure: neurorestoration by phytoestrogens.Journal of neurochemistry · 2014Article
41 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effects of gonadal steroid hormones on dendritic spines were studied in hippocampal neurons that were dissociated and grown in culture for 2-3 weeks. Exposure to estradiol caused up to a twofold increase in dendritic spine density in these neurons. The effect of estradiol was stereospecific and blocked by the steroid antagonist tamoxifen. The estradiol-induced rise in spine density was blocked by the NMDA antagonist APV, but not by the AMPA/KA antagonist DNQX. The estradiol-induced rise in spine density was blocked by the serine/threonine kinase inhibitor H7, but not by the tyrosine kinase inhibitor genestein, and was partially mimicked by PMA, an activator of protein kinase C. Estradiol also caused an increase in the fluorescence intensity of synaptophysin-immunoreactive terminals, corresponding to presynaptic boutons. Finally, estradiol caused a rise in [Ca]i reactivity of the cultured neurons to topical application of glutamate. These studies are the first to examine receptor and second messenger regulation of dendritic spines, and they illustrate the viability of cultured neurons as a powerful test system to address issues related to the regulation of dendritic spine maturation.
Indexed as
Identifiers
8753868PMC6578996W2152110038What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.