ArticleChromosoma1996
Structural changes in oocyte nucleoli of Xenopus laevis during oogenesis and meiotic maturation.
Article in Chromosoma, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- The nucleolus as a polarized coaxial cable in which the rDNA axis is surrounded by dynamic subunit-specific phases.Current biology : CB · 2021Article
- Two splice variants of Nopp140 in Drosophila melanogaster.Molecular biology of the cell · 2002Article
- A novel karyoskeletal protein: characterization of protein NO145, the major component of nucleolar cortical skeleton in Xenopus oocytes.Molecular biology of the cell · 2001Article
- Transient nucleolar localization Of U6 small nuclear RNA in Xenopus Laevis oocytes.Molecular biology of the cell · 2000Article
- Box H and box ACA are nucleolar localization elements of U17 small nucleolar RNA.Molecular biology of the cell · 1999Article
- Role of the box C/D motif in localization of small nucleolar RNAs to coiled bodies and nucleoli.Molecular biology of the cell · 1999Article
- Nucleolar localization elements of Xenopus laevis U3 small nucleolar RNA.Molecular biology of the cell · 1998Article
- Nucleolar localization elements in U8 snoRNA differ from sequences required for rRNA processing.RNA (New York, N.Y.) · 1998Article
- Conserved boxes C and D are essential nucleolar localization elements of U14 and U8 snoRNAs.The EMBO journal · 1998Article
- "Micronucleoli" in the Xenopus germinal vesicle.Chromosoma · 1997Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Immunoelectron microscopy with anti-nucleolin defined substructures within the multiple nucleoli of biosynthetically active stage II-III oocytes and within the nucleoli of relatively quiescent stage VI oocytes of Xenopus laevis. Dense fibrillar components (DFCs) of nucleoli from stage II-III oocytes consisted of nucleolonemas that radiated from a continuous DFC sheath surrounding fibrillar centers (FCs). Discernible granular regions (GRs) were absent in these same nucleoli. Conversely, stage VI oocyte nucleoli displayed compacted DFCs and prominent GRs. Immunofluorescence microscopy then tracked fibrillarin, nucleolin, and condensed DNA through oogenesis and into progesterone-induced meiotic maturation and nuclear breakdown. In stage II-III oocyte nucleoli, fibrillarin was enriched near the FC-DFC boundaries, while nucleolin was distributed throughout these same DFCs. Both proteins were enriched within the compacted DFCs of stage VI oocyte nucleoli. Staining with (DAPI) 4',6-diamidino-2-phenylindole showed condensed DNA within nucleolar FCs of both stage II-III and stage VI oocyte. Upon nuclear breakdown, we found fibrillarin and nucleolin in small particles and in the surrounding cytoplasm. Although we saw no trace of fibrillarin or nucleolin in nuclear remnants prepared just minutes later, DAPI-stained particles remained within these preparations, thus suggesting that FCs were at least slow to disassemble.
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