ArticleMolecular and cellular biochemistry1995
Characterization of the phosphatidylinositol-specific phospholipase C-released form of rat osseous plate alkaline phosphatase and its possible significance on endochondral ossification.
Article in Molecular and cellular biochemistry, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 56 citations in OpenAlex.
- Modulation of TNAP activity and apatite formation in biomimetic matrix vesicles studied byBiochimica et biophysica acta. Biomembranes · 2025Article
- Apigenin Modulates AnxA6- and TNAP-Mediated Osteoblast Mineralization.International journal of molecular sciences · 2022Article
- Article
- Kinetic characterization of a novel acid ectophosphatase from Enterobacter asburiae.Journal of microbiology (Seoul, Korea) · 2016Article
- Effects of GPI-anchored TNAP on the dynamic structure of model membranes.Physical chemistry chemical physics : PCCP · 2015Article
- Proteoliposomes with the ability to transport Ca(2+) into the vesicles and hydrolyze phosphosubstrates on their surface.Archives of biochemistry and biophysics · 2015Article
- Identification and enzymatic characterization of acid phosphatase from Burkholderia gladioli.BMC research notes · 2014Article
- Arsenic binding to proteins.Chemical reviews · 2013Review
- Effects of pH on the production of phosphate and pyrophosphate by matrix vesicles' biomimetics.Calcified tissue international · 2013Article
- Proteoliposomes in nanobiotechnology.Biophysical reviews · 2012Review
- Thermodynamic properties and characterization of proteoliposomes rich in microdomains carrying alkaline phosphatase.Biophysical chemistry · 2011Article
- Assay format as a critical success factor for identification of novel inhibitor chemotypes of tissue-nonspecific alkaline phosphatase from high-throughput screening.Molecules (Basel, Switzerland) · 2010Review
- Kinetic analysis of substrate utilization by native and TNAP-, NPP1-, or PHOSPHO1-deficient matrix vesicles.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2010Article
- Proteoliposomes harboring alkaline phosphatase and nucleotide pyrophosphatase as matrix vesicle biomimetics.The Journal of biological chemistry · 2010Article
- Discovery and validation of a series of aryl sulfonamides as selective inhibitors of tissue-nonspecific alkaline phosphatase (TNAP).Journal of medicinal chemistry · 2009Article
- Streptozotocin-induced diabetes influences the activity of ecto-nucleoside triphosphate diphosphohydrolase 1 of rat osseous plate membranes.Molecular and cellular biochemistry · 2004Article
- Rat osseous plate alkaline phosphatase: effect of neutral protease digestion on the hydrolysis of pyrophosphate and nitrophenylphosphate.Molecular and cellular biochemistry · 2002Article
- Removal from the membrane affects the interaction of rat osseous plate ecto-nucleosidetriphosphate diphosphohydrolase-1 with substrates and ions.The Journal of membrane biologyArticle
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alkaline phosphatase activity was released up to 100% from the membrane by incubating the rat osseous plate membrane-bound enzyme with phosphatidylinositol-specific phospholipase C. The molecular weight of the released enzyme was 145,000 on Sephacryl S-300 gel filtration and 66,000 on PAGE-SDS, suggesting a dimeric structure. Solubilization of the membrane-bound enzyme with phospholipase C did not destroy its ability to hydrolyse PNPP, ATP and pyrophosphate. The hydrolysis of ATP and PNPP by phosphatidylinositol-specific phospholipase C-released enzyme exhibited 'Michaelian' kinetics with K0.5 = 70 and 979 microM, respectively. For pyrophosphate, K0.5 was 128 microM and site-site interactions were observed (n = 1.4). Magnesium ions were stimulatory (K0.5 = 1.5 mM) and zinc ions were a powerful noncompetitive inhibitor (Ki = 6.2 microM) of phosphatidylinositol-specific phospholipase C-released enzyme. Phosphatidylinositol-specific phospholipase C-released alkaline phosphatase was relatively stable at 40 degrees C. However, with increasing temperature from 40-60 degrees C, the enzyme was inactivated rapidly following first order kinetics and thermal inactivation constants varied from 5.08 x 10(-4) min-1 to 0.684 min-1. Treatment of phosphatydilinositol-specific phospholipase C-released alkaline phosphatase with Chellex 100 depleted to 5% its original PNPPase activity. Magnesium (K0.5 = 29.5 microM), manganese (K0.5 = 5 microM) and cobalt ions (K0.5 = 10.1 microM) restored the activity of Chelex-treated enzyme, demonstrating its metalloenzyme nature. The stimulation of Chelex-treated enzyme by calcium ions (K0.5 = 653 microM) was less effective (only 26%) and occurred with site-site interactions (n = 0.7). Zinc ions had no stimulatory effects. The possibility that the soluble form of the enzyme, detected during endochondral ossification, would arise by the hydrolysis of the Pl-anchored form of osseous plate alkaline phosphatase is discussed.
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