ArticleJournal of clinical microbiology1996
Cloning of Brucella abortus gene and characterization of expressed 26-kilodalton periplasmic protein: potential use for diagnosis.
Article in Journal of clinical microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 97 citations in OpenAlex.
- Follow-up of antibody changes in brucellosis patients in Gansu, China.Microbiology spectrum · 2025Article
- A fluorescent bead-based multiplex assay for the detection ofFrontiers in microbiology · 2025Article
- A subunit vaccine based onActa biochimica et biophysica Sinica · 2024Article
- Production and evaluation of anti-BP26 monoclonal antibodies for the serological detection of animal brucellosis.Frontiers in veterinary science · 2024Article
- Development and evaluation of the recombinant BP26 protein-based C-ELISA for human brucellosis diagnosis.Frontiers in microbiology · 2024Article
- Brucellosis detection and the role ofVeterinary world · 2023Review
- Evaluation of the Combined Use of Major Outer Membrane Proteins in the Serodiagnosis of Brucellosis.Infection and drug resistance · 2022Article
- Brucella abortus S19 GFP-tagged vaccine allows the serological identification of vaccinated cattle.PloS one · 2021Article
- Binding of Brucella protein, Bp26, to select extracellular matrix molecules.BMC molecular and cell biology · 2019Article
- Article
- Expression, purification and immunochemical characterization of recombinant OMP28 protein of Brucella species.Open veterinary journal · 2016Article
- Serological diagnostic potential of recombinant outer membrane proteins (rOMPs) from Brucella melitensis in mouse model using indirect enzyme-linked immunosorbent assay.BMC veterinary research · 2015Article
- Limitations of the BP26 protein-based indirect enzyme-linked immunosorbent assay for diagnosis of Brucellosis.Clinical and vaccine immunology : CVI · 2013Article
- Sustained and differential antibody responses to virulence proteins of Brucella melitensis during acute and chronic infections in human brucellosis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2013Article
- Article
- Characterization of periplasmic protein BP26 epitopes of Brucella melitensis reacting with murine monoclonal and sheep antibodies.PloS one · 2012Article
- Systems biology approach predicts antibody signature associated with Brucella melitensis infection in humans.Journal of proteome research · 2011Article
- Evaluation of the recombinant 10-kilodalton immunodominant region of the BP26 protein of Brucella abortus for specific diagnosis of bovine brucellosis.Clinical and vaccine immunology : CVI · 2011Article
- Large scale immune profiling of infected humans and goats reveals differential recognition of Brucella melitensis antigens.PLoS neglected tropical diseases · 2010Article
- P26-based serodiagnosis for Bartonella spp. infection in cats.Comparative medicine · 2008Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brucella spp. are the causative agents of brucellosis in many different hosts, including humans. Most of the serological methods of diagnosis are based on the detection of antilipopolysaccharide antibodies, which makes the differentiation of vaccinated animals from infected animals difficult. By using molecular biology techniques, a gene that encodes a 26-kDa protein (BP26) was isolated from a Brucella abortus S19 genome lambda gt11 library. This protein is in the periplasm of B. abortus and in transformed Escherichia coli. It is exported to the periplasm via a preprotein of 29 kDa with a signal sequence of 28 amino acids. The nucleotide and amino acid sequences of this gene and protein did not show any similarity with those of previously sequenced genes. The use of this protein in Western blotting allowed the differentiation between vaccinated bovines from infected bovines and the detection of infected rams: on the other hand, sera from human patients with active brucellosis were positive, while sera from human patients with chronic brucellosis or without clinical signs were nonreactive. BP26 might be of value as an antigen for serological diagnosis of brucellosis in different mammals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.