ArticleJournal of clinical microbiology1996
Variant colony surface antigenic phenotypes within mycoplasma strain populations: implications for species identification and strain standardization.
Article in Journal of clinical microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 77 citations in OpenAlex.
- Review
- The Performance of Three Immune Assays to Assess the Serological Status of Cattle Experimentally Exposed to Mycoplasma bovis.Veterinary sciences · 2018Article
- Kinetics of Genetic Variation of the Mycoplasma genitalium MG192 Gene in Experimentally Infected Chimpanzees.Infection and immunity · 2015Article
- Characterization of in vivo-acquired resistance to macrolides of Mycoplasma gallisepticum strains isolated from poultry.Veterinary research · 2011Article
- Revised minimal standards for description of new species of the class Mollicutes (division Tenericutes).International journal of systematic and evolutionary microbiology · 2007Article
- Erosive polyarthritis associated with Mycoplasma gateae in a cat.Journal of feline medicine and surgery · 2007Article
- Distinctive repertoire of contingency genes conferring mutation- based phase variation and combinatorial expression of surface lipoproteins in Mycoplasma capricolum subsp. capricolum of the Mycoplasma mycoides phylogenetic cluster.Journal of bacteriology · 2006Article
- Protein and antigenic variability among Mycoplasma hyopneumoniae strains by SDS-PAGE and immunoblot.Veterinary research communications · 2005Article
- Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of Mycoplasma species.BMC infectious diseases · 2005Article
- Cytadherence-deficient mutants of Mycoplasma gallisepticum generated by transposon mutagenesis.Infection and immunity · 2003Article
- Variable surface protein Vmm of Mycoplasma mycoides subsp. mycoides small colony type.Journal of bacteriology · 2002Article
- Coupled phase-variable expression and epitope masking of selective surface lipoproteins increase surface phenotypic diversity in Mycoplasma hominis.Infection and immunity · 2001Article
- Characterization and analysis of a stable serotype-associated membrane protein (P30) of Mycoplasma agalactiae.Journal of clinical microbiology · 2001Article
- Molecular variability of the adhesin-encoding gene pvpA among Mycoplasma gallisepticum strains and its application in diagnosis.Journal of clinical microbiology · 2001Article
- Diagnosis of contagious bovine pleuropneumonia by PCR-laser- induced fluorescence and PCR-restriction endonuclease analysis based on the 16S rRNA genes of Mycoplasma mycoides subsp. mycoides SC.Journal of clinical microbiology · 1999Article
- Development of a recombinant antigen for antibody-based diagnosis of Mycoplasma bovis infection in cattle.Clinical and diagnostic laboratory immunology · 1999Article
- Phase variations of the Mycoplasma penetrans main surface lipoprotein increase antigenic diversity.Infection and immunity · 1999Article
- Molecular biology and pathogenicity of mycoplasmas.Microbiology and molecular biology reviews : MMBR · 1998Review
- Mycoplasma synoviae has two distinct phase-variable major membrane antigens, one of which is a putative hemagglutinin.Infection and immunity · 1997Article
- Adherence of Mycoplasma gallisepticum involves variable surface membrane proteins.Infection and immunity · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Immunobinding assays with mycoplasma colonies on agar plates (immunofluorescence and immunoperoxidase techniques) or with imprints of colonies transferred to solid supports (colony immunoblotting) are widely used as standard diagnostic tests for serological species identification of mycoplasma isolates. However, in light of the high rate of variability of surface antigens in many mycoplasmas, diagnostic data obtained with these techniques require a more critical evaluation. In this report, we demonstrate with some examples that mycoplasma surface variability based on alterations in expression, in size, and in surface presentation of integral and peripheral membrane proteins may lead to misinterpretation of colony immunostaining reactions obtained by using specific monoclonal antibodies as well as conventional diagnostic hyperimmune sera. To more easily identify phenotypically mixed isolates or samples which contain more than one species, we have introduced some minor modifications of the colony immunoblot technique which provide sharp signals of positive as well as negative reactions and enable identification of cryptic epitopes. It is further demonstrated that because of the variability in colony surface antigenic phenotype, mycoplasma strains, including well-established reference and other prototype strains which are used under the same designation in many laboratories, can differ markedly in their antigen profiles and their potentially virulence-related surface properties, since they are usually purified by filter cloning and often propagated by subcultivation of randomly selected agar-grown subpopulations. We conclude from this study that because of this surface variability, the establishment of criteria for standardization of mycoplasma strains and diagnostic antisera is urgently required in order to obtain reproducible results in different laboratories.
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