ArticleJournal of clinical microbiology1996
Genotypic and phenotypic characterization of Escherichia coli isolates from dogs manifesting attaching and effacing lesions.
Article in Journal of clinical microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Epidemiology, Virulence and Antimicrobial Resistance ofMicroorganisms · 2024Article
- A One Health Perspective for Defining and DecipheringComparative medicine · 2021Review
- TheVeterinary and animal science · 2017Article
- Enteropathogenic Escherichia coli (EPEC) infection in association with acute gastroenteritis in 7 dogs from Saskatchewan.The Canadian veterinary journal = La revue veterinaire canadienne · 2016Article
- Enteric bacterial pathogens in children with diarrhea in Niger: diversity and antimicrobial resistance.PloS one · 2015Article
- Identification and characterization of class 1 integrons among atypical enteropathogenic Escherichia coli isolated from children under 5 years of age.Iranian journal of microbiology · 2014Article
- Real-time PCR threshold cycle cutoffs help to identify agents causing acute childhood diarrhea in Zanzibar.Journal of clinical microbiology · 2014Article
- Prevalence of diarrhea-associated virulence genes and genetic diversity in Escherichia coli isolates from fecal material of various animal hosts.Applied and environmental microbiology · 2013Article
- Comparison of rectal swabs and faeces for real-time PCR detection of enteric agents in Rwandan children with gastroenteritis.BMC infectious diseases · 2013Article
- Prevalence and antibiotic resistance patterns of diarrheagenic Escherichia coli isolated from adolescents and adults in Hamedan, Western Iran.Iranian journal of microbiology · 2013Article
- Action of phosphorylated mannanoligosaccharides on immune and hematological responses and fecal consistency of dogs experimentally infected with enteropathogenic Escherichia coli strains.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2013Article
- Ribotyping of EPEC isolates from diarrheal and asymptomatic patients in Iran.Gastroenterology and hepatology from bed to bench · 2012Article
- Phenotypic and genotypic characterization of Escherichia Coli O111 serotypes.Gastroenterology and hepatology from bed to bench · 2011Article
- Associations between antimicrobial resistance phenotypes, antimicrobial resistance genes, and virulence genes of fecal Escherichia coli isolates from healthy grow-finish pigs.Applied and environmental microbiology · 2009Article
- Genotypic and phenotypic profiles of enterotoxigenic Escherichia coli associated with acute diarrhea in Tunis, Tunisia.Current microbiology · 2007Article
- Prevalence of shiga toxin-producing Escherichia coli in a diarrheagenic Tunisian population, and the report of isolating STEC O157:H7 in Tunis.Current microbiology · 2006Article
- The established intimin receptor Tir and the putative eucaryotic intimin receptors nucleolin and beta1 integrin localize at or near the site of enterohemorrhagic Escherichia coli O157:H7 adherence to enterocytes in vivo.Infection and immunity · 2006Article
- Interaction of enteropathogenic and Shiga toxin-producing Escherichia coli and porcine intestinal mucosa: role of intimin and Tir in adherence.Infection and immunity · 2005Article
- Host immune status influences the development of attaching and effacing lesions in weaned pigs.Infection and immunity · 2005Article
- Attaching-effacing bacteria in animals.Journal of comparative pathology · 2005Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thirteen Escherichia coli isolates from dogs manifesting attaching and effacing lesions were characterized genetically with respect to the presence of the following virulence determinants associated with human enteropathogenic E. coli (EPEC): eaeA, encoding the outer membrane protein intimin; eaeB, which is necessary for inducing signal transduction; bfpA, encoding the bundle-forming pilus; and the EAF (stands for EPEC adherence factor) plasmid. These isolates were also analyzed phenotypically with respect to adherence to mammalian cells in vivo and in vitro. Nine of these 13 isolates were found to be eaeA positive by PCR: four of these nine were eaeB positive. The 5' end, but not the 3' end, of the eaeA gene was amplified by PCR when primers derived from the eaeA gene of EPEC were used. Six and eight of these 13 isolates were found to be bfpA positive and EAF positive, respectively. The bfpA gene and EAF locus were found on high-molecular-weight plasmids, whereas the eaeA and eaeB genes were chromosomally located when present. Only one canine E. coli isolate, 4221, which was positive for eaeA, eaeB, bfpA, and EAF, adhered to HEp-2 cells in a localized manner and was positive in the fluorescence actin staining test. The nine eaeA-positive isolates adhered to the mucosal surface of piglet ileal explants and induced some microvillus effacement. However, when tested in experimentally inoculated gnotobiotic piglets, isolate 4221 did not induce attaching and effacing lesions at any level of the intestinal tract. Our results indicate that canine E. coli isolates associated with attaching and effacing lesions share some properties with human EPEC but form a heterogeneous group.
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